高原大棚越冬结球甘蓝游离小孢子培养技术  

Isolated microspore culture technique of overwintering cabbage in plateau greenhouse

作　　者：康少辉 闫凤岐 栗淑芳 申领艳 庞建文 曹雪梅 KANG Shaohui;YAN Fengqi;LI Shufang;SHEN Lingyan;PANG Jianwen;CAO Xuemei(Zhangjiakou Academy of Agricultural Science,Zhangjiakou 075000,Hebei,China)

机构地区：[1]张家口市农业科学院,河北张家口075000

出　　处：《中国瓜菜》2025年第1期196-200,共5页China Cucurbits And Vegetables

基　　金：国家现代农业产业技术体系建设专项(CARS-23-G07);河北省现代农业产业技术体系建设专项(HBCT2024140201,HBCT2024140203)。

摘　　要：游离小孢子培养能够快速获得纯合单倍体,从而有效地缩短杂交后代的繁殖时间,是开展植物育种和基因改良的重要手段。总结了“张甘”系列游离小孢子培养技术,包括从材料播种、实验室准备、选蕾、花蕾消毒、抽提小孢子、离心、出胚培养、胚状体成苗培养、植株移栽等方面内容,以期为结球甘蓝育种者提供参考。“游离小孢子培养技术在甘蓝种质资源创制上的应用研究”成果,于2021年3月通过张家口市科学技术局组织的科技成果鉴定,为国际先进水平,取得河北省科技成果登记证书。Isolated microspore culture could quickly obtain homozygous haploid,thus effectively reducing the reproduction time of hybrid offspring,which was an important means to carry out plant breeding and gene improvement.After years of research and exploration,the team had established a technical system for isolated microspore culture of cabbage in the alpine plateau area.In this paper,the techniques of isolated microspore culture of‘Zhanggan’series were summarized,including material sowing,laboratory preparation,bud selection,bud disinfection,pollen extraction,centrifugation,embryo culture,embryoid seedling culture,plant transplanting and so on,in order to provide reference for cabbage breeders.The results of the application of isolated microspore culture technology in the creation of cabbage germplasm resources were passed the appraisal of scientific and technological achievements organized by Zhangjiakou Science and Technology Bureau in March 2021,which was the international advanced level and obtained the registration certificate of scientific and technological achievements in Hebei province.

关 键 词：结球甘蓝 游离小孢子 培养技术 

分 类 号：S635.1[农业科学—蔬菜学]