杉木梢枯病病原菌Bipolaris fujianensis菌丝体的生物学特性  

Biological characteristics of the mycelium of Bipolaris fujianensis,a novel shoot blight pathogen of Chinese fir

作　　者：薛丽丽 陈春莉 王晓亭 滕剑 黎江南 张清华[1] XUE Lili;CHEN Chunli;WANG Xiaoting;TENG Jian;LI Jiangnan;ZHANG Qinghua(Institute of Forest Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Yangkou State-owned Forest Farm,Shunchang 353299,China;Lianjiang State-owned Forest Farm,Lianjiang 350500,China)

机构地区：[1]福建农林大学森林保护研究所,福建福州350002 [2]福建省洋口国有林场,福建顺昌353299 [3]福建省连江国有林场,福建连江350500

出　　处：《中国森林病虫》2024年第6期12-17,I0002,I0003,共8页Forest Pest and Disease

基　　金：福建省林业科技项目“杉木无性系扦插苗病害成因及综合防治”(2023FKJ24)。

摘　　要：福建平脐蠕孢菌Bipolaris fujianensis是杉木苗梢枯病的新病原真菌,为明确其生物特性,采用生长速率法观察不同温度、光照条件、pH值、培养基、碳源和氮源对其菌丝体生长的影响。结果表明:福建平脐蠕孢菌菌丝体在PDA培养基25℃条件下生长速度为1.61 cm/d,最适生长温度范围为25~30℃,最适宜光照条件是12 h光暗交替,pH值5~13范围内均可正常生长,最适pH值范围为6~9。在不同的培养基上,菌丝体生长差异显著,其中在黄豆、燕麦蔗糖、玉米粉3种培养基上均生长较好,菌落生长速度快;最适碳源为蔗糖和果糖,最适氮源为牛肉浸膏。该结果可为由该病原菌引起的杉木梢枯病的流行规律调查及防治措施制定提供理论基础。Bipolaris fujianensis was a novel pathogenic fungus of Chinese fir shoot blight.To clarify its biological characteristics,the growth rate method was used to observe the effects of different temperatures,light conditions,pH values,media,carbon and nitrogen sources on the growth of the mycelium.The results showed that the mycelium of B.fujianensis grew at a rate of 1.61 cm/d on PDA medium at 25℃,the optimum growth temperature range was 25-30℃,the optimum light condition was an interval 12 h alternative light and dark.It could grow normally within a pH range of 5-13,and the optimum pH value range was 6-9.The growths of mycelium in different media were significantly different,the colony grew better on soybean,oat sucrose and corn flour medium,and the colony growth rates were faster.The most suitable carbon sources were sucrose and fructose,and the most suitable nitrogen source was beef extract.The results can provide a theoretical foundation for researching the epidemic patterns and control methods of Chinese fir shoot blight caused by B.fujianensis.

关 键 词：梢枯病 杉木 福建平脐蠕孢菌 生物学特性 

分 类 号：S763.15[农业科学—森林保护学]