中国东北地区旱柳上的一种锈菌新记录  

A new record species of rust fungi on Salix matsudana in Northeast China

作　　者：田志炫 范淼[2] 王占斌[1] 池玉杰[1,3] TIAN Zhixuan;FAN Miao;WANG Zhanbin;CHI Yujie(College of Forestry,Northeast Forestry University,Harbin 150040,China;Forest Botanical Garden of Heilongjiang Province,Harbin 150046,China;College of Notoginseng Medicine and Pharmacy,Wenshan University,Wenshan 663099,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]黑龙江省森林植物园,黑龙江哈尔滨150046 [3]文山学院三七医药学院,云南文山663099

出　　处：《中国森林病虫》2024年第6期27-31,共5页Forest Pest and Disease

基　　金：东北林业大学双一流专项-自主创新-科技创新团队项目“东北亚锈菌编目、区系研究及数据库建立”(411147021003,411153030301)。

摘　　要：为鉴定发生在哈尔滨市旱柳Salix matsudana上的一种锈病病原,采用光学和扫描电子显微镜观察其夏孢子形态学特征,提取其夏孢子基因组DNA,扩增ITS和LSU基因并测序,依据ITS和LSU串联序列构建系统发育树。系统发育树上该病原菌与铁锈栅锈菌Melampsora ferrinii汇为一支,结合形态学鉴定,确定该病原菌是铁锈栅锈菌Melampsora ferrinii Toome&Aime。该锈菌是东北地区的锈菌新记录种,旱柳是该锈菌在国际首次报道的新寄主植物。To identify the pathogen of rust disease on Salix matsudana,the morphological characteristics of urediospores were observed using both light microscope and scanning electron microscope.The genomic DNA of urediospores were extracted,PCR amplification was performed on the ITS and LSU of ribosomes to further construct a phylogenetic tree.The pathogen was found to cluster with Melampsora ferrinii on the phylogenetic tree.Combined with morphological identification,the pathogen was determined to be M.ferrinii Toome&Aime.M.ferrinii Toome&Aime was a new record species in the Northeast region of China,and S.matsudana was reported as a new host plant for this fungus internationally for the first time.

关 键 词：锈病 旱柳 铁锈栅锈菌 系统发育分析 新记录 

分 类 号：S763.15[农业科学—森林保护学]