有机碳源种类对硫自养短程反硝化的影响  

The impact of different types of organic carbon sources on sulfur autotrophic partial denitrification processes

作　　者：史智炀 周俊才 聂显玲 刘权 李鹏 张辉[1] 陈婉秋 李晓玲[4] SHI Zhiyang;ZHOU Juncai;NIE Xianling;LIU Quan;LI Peng;ZHANG Hui;CHEN Wanqiu;LI Xiaoling(School of Civil Engineering,Chang′an University,Xi′an 710061,China;Jinan Urban and Rural Construction Development Service Center,Jinan 250000,China;Environmental and Social Development Research Center,Chang′an University,Xi′an 710061,China;Key Laboratory of Water Supply and Sewage Engineering,Ministry of Housing and Urban-Rural Development,Chang′an University,Xi′an 710061,China)

机构地区：[1]长安大学建筑工程学院,西安710061 [2]济南市城乡建设发展服务中心,济南250000 [3]长安大学环境与社会发展研究中心,西安710061 [4]长安大学建筑工程学院住建部给排水重点实验室,西安710061

出　　处：《环境工程学报》2024年第11期3228-3237,共10页Chinese Journal of Environmental Engineering

基　　金：陕西省面上自然科学基金(2024JC-YBMS-416)。

摘　　要：本研究以厌氧序批式反应器(anaerobic sequencing batch reactor,ASBR)作为硫自养短程反硝化(sulfur autotrophic partial denitrificationSAPD)的研究体系,以硫代硫酸盐为电子供体。采用批式实验探究了不同有机碳源(甲醇、乙醇、乙酸钠、葡萄糖)对SAPD亚硝酸盐积累的影响,并将批式实验结果应用到ASBR系统,验证其强化效果。当S/N为1时,ASBR系统成功稳定运行,硝酸盐去除率(nitrate removal efficiency,NRE)和亚硝酸盐积累率(nitrite accumulation rate,NAE)分别达到了95.89%和52.40%。C/N比为0.5的碳源批式实验结果显示亚硝酸盐的积累效果为:乙酸钠>葡萄糖>甲醇≈乙醇>无碳源,其中乙酸钠对亚氮积累的提升效果最为显著,NAE由50.52%上升至61.09%,其中硫单质生成率由40.43%下降至30.21%。将批式实验结果应用到ASBR中,乙酸钠作用使系统对硝氮(NO_(3)^(−)-N)的处理效果和亚氮(NO_(2)^(−)-N)积累能力进一步提升,NAE从65.00%提高到了73.70%,且NRE也从80.6%提高到了89.15%。高通量测序结果可知,乙酸钠的投加使得SAPD系统中变形菌门(Proteobacteria)丰度显著增加,而厚壁菌门(Fimicutes)活性受到抑制。硫杆菌属Thiobacillus相对丰度由6.83%上升至10.32%,Terrimonas相对丰度由10.68%下降到1.20%。总的来说,低浓度有机物的投加可以提升SPAD工艺的整体效果。The study investigated sulfur autotrophic partial denitrification(SAPD)process by utilizing an anaerobic sequencing batch reactor(ASBR),with thiosulfate serving as the electron donor.The effects of different organic carbon sources(methanol,ethanol,sodium acetate,glucose)on SAPD nitrite accumulation were investigated by batch experiments,and then the results of batch experiments were applied to ASBR system to verify the enhancement effect.When the S/N ratio was 1,the ASBR system successfully operated,achieving a nitrate removal efficiency(NRE)of 95.89%and a nitrite accumulation efficiency(NAE)of 52.40%.Results from batch experiments with a C/N ratio of 0.5 indicated that the effectiveness of nitrite accumulation was as follows:sodium acetate>glucose>methanol≈ethanol>no carbon source.Among these,sodium acetate had the most significant enhancement on nitrite accumulation,increasing the NAE from 50.52%to 61.09%,while the elemental sulfur production rate decreased from 40.43%to 30.21%.The optimization results in ASBR show that,the addition of sodium acetate further enhanced the NO_(3)^(−)-N removal and NO_(2)^(−)-N accumulation ability.The NAE increased from 65.00%to 73.70%,and the NRE also improved from 80.6%to 89.15%.High-throughput sequencing results indicated that the addition of sodium acetate significantly increased the abundance of Proteobacteria in the SAPD system,while the activity of Firmicutes was inhibited.The relative abundance of Thiobacillus increased from 6.83%to 10.32%,and the relative abundance of Terrimonas decreased from 10.68%to 1.20%.Overall,the addition of low concentrations of organic matter can enhance the overall effectiveness of the SAPD process.

关 键 词：硫自养短程反硝化 硝酸盐去除 有机碳源 NO_(2)−-N积累 微生物群落 

分 类 号：X703[环境科学与工程—环境工程]