外源性黄体生成素对玻璃化冻存颗粒细胞的影响及机制研究  

Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism

作　　者：陈杰 高尚[1] 张少杰 杨喜 李琨 史君 王星 CHEN Jie;GAO Shang;ZHANG Shao-jie;YANG Xi;LI Kun;SHI Jun;WANG Xing(Department of Anatomy of Basic Medical School,Inner Mongolia Medical University,Hohhot Inner Mongolia 010110,China;Stem Cell Research Center,Inner Mongolia Medical University,Hohhot Inner Mongolia 010110,China;Digital Medicine Center of Basic Medical School,Inner Mongolia Medical University,Hohhot Inner Mongolia 010110,China;Department of Physiology of Basic Medical School,Inner Mongolia Medical University,Hohhot Inner Mongolia 010110,China)

机构地区：[1]内蒙古医科大学基础医学院人体解剖学教研室,内蒙古呼和浩特010110 [2]内蒙古医科大学干细胞研究中心,内蒙古呼和浩特010110 [3]内蒙古医科大学基础医学院数字医学中心,内蒙古呼和浩特010110 [4]内蒙古医科大学基础医学院生理学教研室,内蒙古呼和浩特010110

出　　处：《局解手术学杂志》2025年第1期1-5,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(81860382,81860383);内蒙古自然科学基金(2020MS03061,2020MS08109,2019MS08017,2019MS08139,2022SHZR0151);内蒙古自治区科技发展计划项目(2019GG158);内蒙古自治区卫生健康委医疗卫生科技计划项目(202201217);内蒙古自治区高等学校青年科技英才项目(NJYT22009);内蒙古医科大学科研重点项目(YKD2021ZD011)。

摘　　要：目的 观察外源性黄体生成素(LH)对玻璃化冻存颗粒细胞的影响及其作用机制。方法 取4周龄青春期前小鼠颗粒细胞,分为新鲜组(不进行玻璃化冻存)、玻璃化冻存组(按玻璃化冻存程序处理)和LH组(在玻璃化冻存组的基础上添加0.3 IU/mL LH干预)。进行颗粒细胞计数,采用Realtime PCR及Western blot检测各组Foxl2、PI3K、AKT及mTOR mRNA和蛋白的表达变化。结果 与玻璃化冻存组相比,LH组颗粒细胞总数显著增加(P<0.05),Foxl2、PI3K、AKT、mTOR mRNA及蛋白的表达显著升高(P<0.05)。结论 外源性LH干预有利于冻存颗粒细胞的存活,其机制可能与激活PI3K/AKT/mTOR通路有关。Objective To investigate the effect of exogenous luteinizing hormone(LH) on vitrification granulosa cells and its mechanisms.Methods The granulosa cells of 4-week-old preadolescent mice were selected and divided into the fresh group(without vitrification procedure),the vitrification group(treated according to vitrification procedure) and the LH group(treated with 0.3 IU/L of LH intervention on the basis of the vitrification group).Granulocyte count was performed,and Realtime PCR and Western blot were used to detect the expression changes of Foxl2,PI3K,AKT and mTOR mRNAs and proteins in each group.Results Compared with the vitrification group,the total number of granulosa cells in the LH group was significantly increased(P<0.05),and the expression of Foxl2,PI3K,AKT and mTOR mRNAs and proteins were elevated(P<0.05).Conclusion The intevention of exogenous LH is beneficial for the survival of vitrification granulosa cells,and the mechanism may be related to the activation of PI3K/AKT/mTOR pathway.

关 键 词：颗粒细胞 玻璃化冻存 黄体生成素 FOXL2 PI3K/AKT/mTOR通路 

分 类 号：R711.75[医药卫生—妇产科学]