茶树应答茶小绿叶蝉为害的蛋白质组分析  

Proteomics of Tea Plant in Response to Infestation by Empoasca vitis

作　　者：王芬 喻佩 翟媛 李传刚 罗思妤 裴会敏 WANG Fen;YU Pei;ZHAI Yuan;LI Chuangang;LUO Siyu;PEI Huimin(School of Biological Science and Agriculture,Qiannan Normal University for Nationalities,Duyun,Guizhou 558000,China)

机构地区：[1]黔南民族师范学院生物科学与农学院,贵州都匀558000

出　　处：《福建农业学报》2024年第10期1179-1188,共10页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32260080);贵州省教育厅项目(QNYSKYTD2018006);黔南民族师范学院项目(2019xjg0303、2018xjg0520、 QNSY2018ZJ006、QNYSXXK2018005)

摘　　要：【目的】探明都匀毛尖本地种茶树响应茶小绿叶蝉为害相关的蛋白质。【方法】以被茶小绿叶蝉侵染0、12、24、36、48 h的都匀毛尖本地种茶树叶片为材料,利用串联质谱标签(tandem mass tag,TMT)结合液相色谱-串联质谱(LC-MS/MS)技术对茶小绿叶蝉侵染茶树叶片后的蛋白质进行定性定量分析。【结果】共鉴定出2893个蛋白质,0 h与12 h、24 h、36 h、48 h之间分别有0、1、848、849个差异蛋白质,共有2622个蛋白质被注释,其中2360个蛋白注释到GO数据库,1232个注释到KEGG数据库。GO和KEGG分析表明茶小绿叶蝉侵染茶树时可能通过差异蛋白质4-二磷酸胞基-2-c-甲基-d-赤藓糖醇激酶、法尼基二磷酸合成酶、香叶基二磷酸合成酶、香叶基香叶基二磷酸合成酶、过氧化物酶、果胶酯酶、丙二烯氧化环化酶、倍半萜类、三萜类、单萜类、二萜类化合物以及倍半萜和三萜生物合成途径、萜类骨架生物合成途径和单萜生物合成途径来抵御害虫的侵染。【结论】筛选出4个差异蛋白质(4-二磷酸胞基-2-c-甲基-d-赤藓糖醇激酶、法尼基二磷酸合成酶、香叶基二磷酸合成酶和香叶基香叶基二磷酸合成酶)和萜类化合物可能在应答防御小绿叶蝉侵害时具有重要的作用。研究结果可为揭示茶树应答茶小绿叶蝉为害的分子机制提供理论依据。【Objective】Proteins related to the response of Duyun Maojian native tea plant infested by Empoasca vitis were studied.【Method】Leaves of Duyun Maojian native tea plant were artificially infested by E.vitis Göthe for 0,12,24,36,and 48 h.Tandem mass tag combined with liquid chromatography-tandem mass spectrometry was applied to detect the proteins generated in the leaves by the infestation.【Results】The chemical analysis identified 2893 proteins that included 0,1,848,and 849 differential expression proteins(DEPs)in the leaf specimens sampled at the time periods that lasted from 0 h to 12 h,24 h,36 h,and 48 h,respectively.Of the 2622 annotated proteins,2360 were explained to the GO database and 1232 to the KEGG database.The GO and KEGG analyses indicated that the tea plants might be resistant to E.vitis infestation through the enzymes,such as CMK,FPPS,GPPS,GGPPS,peroxidase,pectinesterase,and allene-oxide cyclase,the biosynthesis of sesquiterpenoid,triterpeoid,monoterpenoid,and diterpenoid,and/or the biosynthesis pathways of sesquiterpenoid,triterpenoid,terpenoid backbone,and monoterpenoid.【Conclusion】Four DEPs,i.e.,CMK,FPPS,GPPS,and GGPPS,and terpenoids might play important roles in the response and defense of the tea plant against E.vitis Göthe.

关 键 词：茶树 茶小绿叶蝉 蛋白质组 TMT 差异蛋白 

分 类 号：S435[农业科学—农业昆虫与害虫防治]