野生金顶侧耳无性阶段分子鉴定和显微形态观察  

Molecular identification and microscopic morphological observation of the asexual stage of wild Pleurotus citrinopileatus

作　　者：秦琴 郭永杰[2,3] 韦静宜 杜习慧[1] QIN Qin;GUO Yongjie;WEI Jingyi;DU Xihui(College of Life Sciences,Chongqing Normal University,Chongqing 401331,China;Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,Yunnan,China;University of Chinese Academy of Sciences,Beijing 100049,China;Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]重庆师范大学生命科学学院,重庆401331 [2]中国科学院昆明植物研究所中国西南野生生物种质资源库,云南昆明650201 [3]中国科学院大学,北京100049 [4]重庆市农业科学院,重庆401329

出　　处：《菌物学报》2024年第12期31-41,共11页Mycosystema

基　　金：国家自然科学基金(32270023);重庆市自然科学基金(CSTB2022NSCQ-LZX0035);重庆市教委科学技术研究项目(KJQN202200562,KJQN202300503);重庆市食用菌种质资源库项目(WSWZZ2020001)。

摘　　要：基于采自昆明植物园生长在云南松腐朽树桩上的一簇野生菌丝,运用分子系统发育研究确定其为侧耳属真菌金顶侧耳Pleurotuscitrinopileatus,借助体视显微镜和光学显微镜分别观察到金顶侧耳无性菌丝和无性孢子的形态特征。显微观察结果表明,该金顶侧耳菌株的无性菌丝体由具有锁状联合的双核菌丝占主体,并具有3种无性孢子形成方式,第一种是由双核菌丝侧面分化出短且无分枝的分生孢子梗,并在其顶部形成球形的双核分生孢子,直径为1.50–4.57μm;第二种是由双核菌丝侧枝分化产生椭圆形的双核粉孢子,直径为1.17–3.99μm,或圆柱形的双核粉孢子,大小为2.78–5.40×1.57–3.03μm,第三种是由单核菌丝侧枝分化产生链状圆柱形单核粉孢子,大小为3.71–6.70×3.60–4.02μm。本研究首次对金顶侧耳无性菌丝和无性孢子的形态特征进行了描述和讨论。A wild mycelium sample growing on rotten stump of Pinus yunnanensis collected from the Kunming Botanical Garden in Yunnan Province was identified as Pleurotus citrinopileatus by using molecular phylogenetic method.Morphological characteristics of asexual mycelium and spores were observed under stereomicroscope and light microscope.It was observed that asexual mycelium of P.citrinopileatus were dominated by dikaryotic hyphae with clamp connections and produced asexual spores in three ways:(1)spherical binucleate conidia with size ranging from 1.50 to 4.57μm formed from short and unbranched conidiophores laterally differentiated from dikaryotic hyphae;(2)elliptical binucleate oidia with size ranging from 1.17 to 3.99μm and cylindrical binucleate oidia with size being 2.78-5.40×1.57-3.03μm formed from lateral branches of dikaryotic hyphae;(3)cylindrical uninucleate oidia with size being 3.71-6.70×3.60-4.02μm formed in chains from lateral monokaryotic branches of hyphae.The microscopic characters of asexual mycelium and spores were described and discussed in detail for the first time,with attached figures or drawing.

关 键 词：侧耳属 无性生殖 分生孢子 粉孢子 形态特征 

分 类 号：S64[农业科学—蔬菜学]