叶黄素乳液凝胶3D打印间隔多层结构的构建及释放特性研究  

Construction and Release Characteristics of 3D Printed Spaced Multi-layer Structure of Lutein Emulsion Gel

作　　者：邓帅 赵明慧 郝春雪 李鸣 李大婧 冯蕾[1,2] 张钟元 徐亚元 DENG Shuai;ZHAO Minghui;HAO Chunxue;LI Ming;LI Dajing;FENG Lei;ZHANG Zhongyuan;XU Yayuan(College of Food and Biological Engineering,Jiangsu University,Zhenjiang 212000,China;Agricultural Products Processing Research Institute,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;School of Life Sciences,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212000 [2]江苏省农业科学院农产品加工研究所,江苏南京210014 [3]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《食品工业科技》2025年第3期133-142,共10页Science and Technology of Food Industry

基　　金：国家自然科学基金-青年科学基金(32302127);江苏省农业科技自主创新资金项目(CX(22)3065)。

摘　　要：通过流变特性和3D打印特性评估,筛选叶黄素负载层和空载层乳液凝胶的制备参数,运用双喷头3D打印技术将叶黄素负载层与空载层间隔交错打印,构建不同间隔多层结构的3D打印凝胶体系,探究间隔多层结构的间隔层数和空载层定位对叶黄素的释放特性和生物可及性的影响。结果表明,通过提升油相体积分数、分离乳清蛋白(Whey Protein Isolate,WPI)浓度以及叶黄素载量,可以有效地提高叶黄素乳液凝胶的表观黏度、储能模量和损耗模量,同时降低蠕变恢复应变,增强叶黄素乳液凝胶的黏弹性和抵抗形变的能力,从而显著提升了叶黄素乳液凝胶的打印精确度和稳定性;当油相体积分数为15%、WPI浓度为10%和叶黄素载量为1.5%时,叶黄素乳液凝胶的3D打印效果最佳,打印精确性和稳定性分别为96.94%和97.60%。经体外模拟消化发现,间隔多层结构设计可有效改变叶黄素的释放行为,显著降低了叶黄素在胃消化阶段的释放率,从21.61%最低降至7.26%,使叶黄素在肠消化阶段表现出一定的时滞性,并显著提高叶黄素的生物可及性,最高达到了47.97%。本研究将为解决叶黄素生物可及性低的问题和运载工具的设计提供新的思路和理论依据。In this study,3D printing technology with double nozzles was applied to print lutein emulsion gels(lutein-loaded layer)and corresponding unloaded emulsion gels in an interlaced manner.The preparation parameters of lutein-loaded and unloaded emulsion gels were screened by evaluating the rheological properties and 3D printing characteristics.3D printed gel systems with different intervals of multilayers structures were constructed to explore the effects of the number of spacer layers and the positioning of empty layers in spaced multi-layer structures on the release characteristics and bioaccessibility of lutein.The results indicated that increasing the oil phase volume fraction,separating the concentration of whey protein isolate(WPI),and loading of lutein could effectively enhance the apparent viscosity,storage modulus,and loss modulus of lutein emulsion gels.Simultaneously,it reduced creep recovery strain,strengthened the viscoelasticity of lutein emulsion gels,and improved their resistance to deformation,thereby significantly enhanced the printing accuracy and stability of lutein emulsion gels.When the oil phase volume fraction was 15%,the WPI concentration was 10%and the lutein loading was 1.5%,the lutein emulsion gel showed the best 3D printing results,with printing accuracy and stability of 96.94%and 97.60%,respectively.After simulated digestion in vitro,the interval multi-layer structure could effectively change the release behavior of lutein,significantly reducing the release rate of lutein in the gastric digestion stage from 21.61%to 7.26%at the lowest.Furthermore,the release curve of lutein showed a certain time lag in the intestinal digestion stage,and significantly improving the bioaccessibility of lutein,reaching a maximum of 47.97%.The project will provide new ideas and theoretical basis for solving the bottleneck problem of lutein low bioaccessibility and the design of delivery vehicles.

关 键 词：叶黄素 乳液凝胶 间隔多层结构 释放特性 生物可及性 

分 类 号：TS201.2[轻工技术与工程—食品科学]