三叶斑潜蝇地理种群间的耐热性及Hsp64.9表达研究  

Differences in thermal tolerance and the expression of Hsp64.9 between geographical populations of Liriomyza trifolii

作　　者：常亚文 仲玲 闫玉卿 赵婧雅 龚伟荣 杜予州 CHANG Ya-Wen;ZHONG Ling;YAN Yu-Qing;ZHAO Jing-Ya;GONG Wei-Rong;DU Yu-Zhou(College of Plant Protection&Institute of Applied Entomology,Yangzhou University,Yangzhou 225009,China;Plant Protection and Quarantine Station of Jiangsu Province,Nanjing 210036,China;Joint Laboratory of International Cooperation in Agriculture and Agricultural Products Safety,Ministry of Education,Yangzhou 225009,China)

机构地区：[1]扬州大学植物保护学院暨应用昆虫研究所,扬州225009 [2]江苏省植物保护植物检疫站,南京210036 [3]教育部农业与农产品安全国际合作联合实验室,扬州225009

出　　处：《应用昆虫学报》2024年第5期944-958,共15页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金青年项目(32202275);国家重点研发计划项目(2022YFC2601100);江苏现代农业产业技术体系建设项目(JATS[2023]315)。

摘　　要：【目的】明确三叶斑潜蝇Liriomyza trifolii不同地理种群的高温耐受性和重要热激蛋白基因Hsp64.9的表达模式,探究三叶斑潜蝇地理种群间温度适应性差异的机制。【方法】以三叶斑潜蝇海南和江苏种群为研究对象,分别测定其成虫和蛹在不同高温条件(41-46℃,温度梯度1℃)胁迫1 h和同一温度不同胁迫时长处理(39、42和45℃,处理时长0.25、0.5、1、2和4 h)下的存活率和羽化率,并比较基因Hsp64.9的表达差异。【结果】江苏和海南种群成虫的存活率分别在42和43℃开始显著降低(江苏种群:F_(6,14)=296.459,P<0.001;海南种群:F_(6,14)=255.810,P<0.001),且不同温度条件下,海南种群成虫的存活率显著高于江苏种群(P<0.05)。在39℃胁迫时长短于4 h及42和45℃胁迫时长短于1 h处理下,2个种群成虫的存活率无显著差异(P>0.05),随着胁迫时长的延长,海南种群成虫存活率显著高于江苏种群(P<0.05),除了存活率为0的45℃下2和4 h处理。海南种群蛹的羽化率在42、44和46℃显著高于江苏种群(42℃:t=﹣4.234,P=0.013;44℃:t=﹣5.567,P=0.005;46℃:t=﹣4.000,P=0.016)。同一温度不同胁迫时长处理下,除了42℃胁迫时长1 h外(t=-4.243,P=0.013),2个种群蛹的羽化率均没有显著差异(P>0.05)。海南种群成虫和蛹在不同温度及同一高温不同胁迫时长处理下,除了成虫44℃处理1 h(t=﹣0.475,P=0.659)和蛹45℃处理0.5 h(t=2.723,P=0.053)外,基因Hsp64.9的相对表达量均显著高于江苏种群。【结论】三叶斑潜蝇不同地理种群间的耐热性存在显著差异,Hsp64.9的差异表达可能是海南种群耐热性高于江苏种群的原因。[Aim]To investigate differences in the high temperature tolerance and expression of the heat shock protein gene Hsp64.9 between different geographical populations of Liriomyza trifolii,and investigate the mechanism of temperature adaptation in this species.[Methods]Adults and pupae from the Hainan and Jiangsu populations of L.trifolii were exposed to different high temperatures for different periods.Differences in heat tolerance between these geographical populations were then assessed by comparing adult survival rates and pupal emergence rates.The expression of Hsp64.9 in adults and pupae from each population were also measured and compared.[Results]The survival rate of both the Jiangsu and Hainan populations began to decrease significantly at 42 and 43℃(Jiangsu population:F6,14=296.459,P<0.001;Hainan population:F6,14=255.810,P<0.001),respectively,but the survival rate of the Hainan population was significantly higher than that of the Jiangsu population(P<0.05).There was no significant difference in survival rate between the two populations following exposure to 39℃for less than 4 h,or exposure to 42 or 45℃for less than 1 h(P>0.05).The survival rate of adults from the Hainan population was significantly higher than that of those from the Jiangsu population following longer durations of exposure to high temperatures(P<0.05).The emergence rate of the Hainan population was also significantly higher than that of Jiangsu population after exposure to 42,44 and 46℃for 1 h(42℃:t=﹣4.234,P=0.013;44℃:t=﹣5.567,P=0.005;46℃:t=﹣4.000,P=0.016),but duration of exposure to high temperature had no significant effect on the emergence rate of pupae from these populations(P>0.05).The expression of Hsp64.9 in adults and pupae from the Hainan population was significantly higher than that of those from the Jiangsu population under all treatment combinations(P<0.05).[Conclusion]The Hainan population of L.trifolii is significantly more tolerant to high temperatures than the Jiangsu population,an adaptation that,at leas

关 键 词：三叶斑潜蝇 地理种群 高温处理 耐热性 Hsp64.9 

分 类 号：S433[农业科学—农业昆虫与害虫防治]