非靶标害虫二化螟为害抗褐飞虱水稻诱导植物挥发物变化对靶标害虫抗性的影响  

Effect of changes in volatiles from resistant rice varieties damaged by non-target insect pests on Nilaparvata lugens

作　　者：王兴云 张新强 刘瑶瑶 王璐颖 周亚丽 薛爽 何玲敏 王景顺[1,3] WANG Xing-Yun;ZHANG Xin-Qiang;LIU Yao-Yao;WANG Lu-Ying;ZHOU Ya-Li;XUE Shuang;HE Ling-Min;WANG Jing-Shun(College of Biological and Food Engineering,Anyang Institute of Technology,Anyang 455000,China;Postdoctoral Innovation Practice Base,Anyang Institute of Technology,Anyang 455000,China;Field Scientific Observation and Research Station of Forest Pests in Taihang Mountain of Henan Province,Anyang 455000,China)

机构地区：[1]安阳工学院生物与食品工程学院,安阳455000 [2]安阳工学院博士后创新实践基地,安阳455000 [3]河南省太行山林业有害生物野外科学观测研究站,安阳455000

出　　处：《应用昆虫学报》2024年第5期970-979,共10页Chinese Journal of Applied Entomology

基　　金：国家青年自然科学基金(31802007);河南省科技攻关项目(242102110263);中国博士后面上项目资助(2020M672204);河南省博士后科研项目启动经费(201902042);安阳工学院博士后科研启动基金(BHJ2019002);河南省新农科研究与实践改革项目(2020JGLX144);河南省高等学校青年骨干培养计划(2020GGJS232);安阳工学院“十百千品牌提升计划”和“百千万英才提升计划”。

摘　　要：【目的】植食性昆虫之间存在复杂的种间作用,而虫害诱导的植物挥发物在昆虫互作中起着关键作用。【方法】本研究通过顶空动态收集和气相色谱-质谱联用仪(GC-MS)分析了抗褐飞虱Nilaparvata lugens水稻品种Mudgo和感虫品种TN1分别被非靶标害虫二化螟Chilo suppressalis为害后的挥发物组成,共鉴定到115种挥发物,并筛选出其中具有显著差异的挥发物。使用Y型嗅觉仪测定了靶标害虫褐飞虱对非靶标害虫二化螟为害诱导的20种水稻挥发物(浓度分别为2和200μL/mL,溶剂为正己烷)的行为反应。【结果】Y型嗅觉仪结果表明,水稻Mudgo被二化螟为害后显著增加的挥发物中,正乙醛、2-庚酮、β-月桂烯、芳樟醇、十四烷、正十五烷、正十六烷、正十七烷、α-蒎烯、D-柠檬烯和β-石竹烯11种挥发物对褐飞虱有显著的吸引作用(P<0.05),而苯乙醛、2-壬酮、萘、水杨酸甲酯和十三烷5种挥发物对褐飞虱有驱避作用。结果揭示了抗性和感虫水稻品种被二化螟为害后所释放的挥发物存在显著差异,并且对靶标害虫褐飞虱有一定的吸引作用,提示非靶标害虫为害可能会导致植物对靶标昆虫拒异性降低。【结论】研究结果丰富了寄主植物挥发物在植食性害虫种间作用关系理论,为利用植物挥发物防治害虫提出新的防治策略具有重要实践意义。[Aim]Herbivorous insects have complex interspecific interactions in which herbivore-induced plant volatiles(HIPVs)play an important role.[Methods]In this study,a dynamic headspace collection system,together with gas chromatography-mass spectrometry(GC-MS),were used to compare the effect of volatiles collected from a resistant rice cultivar(Mudgo)and a susceptible rice cultivar(TN1),on the brown planthopper(BPH)Nilaparvata lugens.In addition,the effect of volatiles from each of the above rice varieties that had been damaged by a non-target pest,the rice striped stem borer (SSB) Chilo suppressalis, were also investigated. GC-MS identified a total of 115 volatiles. The behavioral responses of brownplanthoppers to 20 of these at concentrations of 2 and 200 μL dissolved in 1 mL of normal hexane were investigated using aY-tube olfactometer. [Results] This showed that 11 volatiles (i.e., Hexanal, 2-Heptanone, β-Myrcene, Linalool, Tetradecane,Pentadecane, Hexadecane, Heptadecane, α-Pinene, D-limonene and β-Caryophyllene) were significantly more attractive to theBPH than the control, and that the amount of these 11 volatiles significantly increased after damage by SSB (P < 0.05). Incontrast, the other 5 volatiles (Benzeneacetaldehyde, 2-Nonanone, Naphthalene, Methyl salicylate and Tridecane) wererepellent to the BPH. These results reveal that damage to rice plants by the SSB caused significant changes in the volatilesreleased from both resistant, and susceptible, rice cultivars. Furthermore, volatiles collected from rice plants that had beendamaged by the SSB were significantly more attractive effect to the BPH, indicating that a non-target insect pest canpotentially affect the occurrence of a targeted insect pest. These results also suggest that plants damaged by non-target insectpests may have lower resistance to target insects. [Conclusion] These findings provide new information on the interactionbetween plant volatiles and insect species, and have important practical implications for using natural plant volatiles

关 键 词：抗性水稻品种 非靶标害虫二化螟 靶标害虫褐飞虱 植食性害虫种间作用 虫害诱导的植物挥发物 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]