不同浓度噻虫嗪对点蜂缘蝽若虫体内保护酶和解毒酶活性的影响  

Effects of different concentrations of thiamethoxam on the activitiy of protective and detoxification enzymes inRiptortus pedestris

作　　者：王自杰 杨芳哲 柳添杰 田鑫月 王月英 史树森 高宇 WANG Zi-Jie;YANG Fang-Zhe;LIU Tian-Jie;TIAN Xin-Yue;WANG Yue-Ying;SHI Shu-Sen;GAO Yu(Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences,Observation and Experiment Station of Crop Pests of Jiamusi,Ministry of Agriculture,Jiamusi 154007,China;College of Plant Protection,Key Laboratory of Soybean Diseases and Pests Control in Ministry of Agriculture and Rural Affairs,Jilin Agricultural University,Changchun 130118,China;SuzhouAcademy of Agricultural Sciences,Suzhou234099,China)

机构地区：[1]黑龙江省农业科学院佳木斯分院,农业部佳木斯作物有害生物科学观测实验站,佳木斯154007 [2]吉林农业大学植物保护学院,农业农村部大豆病虫害防控重点实验室,长春130118 [3]宿州市农业科学院,宿州234099

出　　处：《应用昆虫学报》2024年第5期1037-1047,共11页Chinese Journal of Applied Entomology

基　　金：黑龙江省省属科研院所科研业务费(CZKYF2023-1-B005);黑龙江省重点研发计划(GA22B014);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-04)。

摘　　要：【目的】探明不同浓度噻虫嗪胁迫点蜂缘蝽Riptortus pedestris F_(0)代3龄若虫后,其体内和F_(1)代3龄若虫体内保护酶和解毒酶活性的变化。【方法】采用药液浸渍法,将清水浸成的“肾型”大豆籽粒,浸入25%噻虫嗪水分散粒剂(Water dispersible granule,WG)用清水配置成LC_(10)(5.2mg/L)、LC_(20)(11.2mg/L)、LC_(30)(19.6mg/L)、LC_(40)(31.6mg/L)和LC_(50)(49.4mg/L)药液20s,设清水对照处理。喂食点蜂缘蝽F。代3龄若虫24h后,分别将各处理存活的若虫,一部分测定其体内过氧化物酶(Peroxidase,POD)、过氧化氢酶(Catalase,CAT)、超氧化物歧化酶(Superoxide dismutase,SOD)乙酰胆碱酯酶(Acetylcholinesterase,AChE)、谷胱甘肽转移酶(Glutathione S-transferase,GST)和羧酸酯酶(Carboxylesterase,CarE)的酶活性变化;另一部分放入养虫笼中喂食清水处理的大豆籽粒,饲养至F_(1)代若虫发育至3龄时,测定其体内POD、CAT、SOD、AChE、GST和CarE的酶活性变化【结果】不同浓度噻虫嗪处理后,F_(0)代若虫体内POD、CAT、SOD、GST及CarE活性均高于对照组;F_(1)代若虫体内POD、AChE、GST和CarE的酶活性高于对照组。所有浓度处理下,点蜂缘蝽F_(1)代若虫体内POD、GST和CarE的酶活性均高于F_(0)代;在LC_(20)-LC_(50)浓度处理,点蜂缘蝽F_(1)代若虫体内AChE的酶活性均高于F_(0)代。点蜂缘蝽F_(1)和F_(1)代若虫体内保护酶和解毒酶的活性与噻虫嗪浓度呈正相关。【结论】在噻虫嗪胁迫下,F_(0)代若虫通过提高体内POD、CAT、SOD、GST和CarE的酶活性进行解毒代谢,F_(0)代若虫则通过提高POD、AChE、GST和CarE活性进行解毒代谢。POD、AChE、GST和CarE酶活性的增加可能是点蜂缘蝽F_(1)代若虫产生抗药性的主要原因。[Aim]To measure changes in the activity of protective and detoxifying enzymes in the F_(0)and the F_(1)generations of the 3rd instar of Riptortus pedestris nymphs exposed to different concentrations of thiamethoxam.[Methods]Reniform soybean seeds soaked in water were immersed in 25%thiamethoxam WG that had been diluted with different amounts of water to obtain LC_(10)(5.2 mg/L),LC_(20)(11.2 mg/L),LC_(30)(19.6 mg/L),LC_(40)(31.6 mg/L)and LC_(50)(49.4 mg/L)solutions.Water was the control.F_(0)nymphs of R.pedestris were fed the treated seeds for 24 hours after which changes in the activity of peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),acetylcholinesterase(AChE),glutathione S-transferase(GST)and carboxylesterase(CarE)in surviving nymphs was measured.Another group of nymphs was placed in cages and fed soybean seeds treated with water until Fi generation nymphs had developed to the 3rd instar,after which changes in the activity of POD,CAT,SOD,AChE,GST,and CarE were determined in vivo.[Results]After treatment with different concentrations of thiamethoxam,the POD,CAT,SOD,GST and CarE activity of Fo generation nymphs was higher than that of the control group.POD,AChE,GST and CarE activity of F,larvae were also higher than that of the control group.The POD,GST and CarE activity of F_(1)larvae was higher than those of F_(0)larvae for all treatment concentrations.AChE enzyme activity of F_(1) larvae was higher than that of Fo larvae for concentrations in the range of LC_(20)-LC_(50).Protective and detoxifying enzyme activity in Fo and Fi larvae was positively correlated with thiamethoxam concentration.[Conclusion]Fo R.pedestris nymphs detoxify thiamethoxam by increasing their POD,CAT,SOD,GST and CarE enzyme activity,whereas Fi nymphs detoxify thiamethoxam by increasing their POD,AChE,GST and CarE activity.The observed changes in POD,AChE,GST and CarE enzyme activity may be the mechanism responsible for pesticide resistance in R.pedestris F_(1) nymphs.

关 键 词：点蜂缘蝽 噻虫嗪 保护酶 解毒酶 抗药性 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]