蓼蓝齿胫叶甲肠道微生物多样性研究  

Diversity of the gut microbiota of Gastrophysa atrocyanea

作　　者：王毛 贺宏 张云书 肖敏 魏真真 郭静微 陈松[1] 陈庆东[1] 肖金鑫 汪东宁 王光旭 WANG Mao;HE Hong;ZHANG Yun-Shu;XIAO Min;WEI Zhen-Zhen;GUO Jing-Wei;CHEN Song;CHEN Qing-Dong;XIAO Jin-Xin;WANG Dong-Ning;WANG Guang-Xu(Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,MOA Key Laboratory of Integrated Management of Pests on Crops in Southwest China,Chengdu 610066,China;Aba State Institute of Agricultural Sciences,Maerkang 624000,China;Aba State Academy of Ecological Conservation and Development,Wenchuan 623000,China;Agricultural Product Quality Inspection and Monitoring Center of Aba,Maerkang 624011,China;Plant Protection and Inspection Station of Aba,Maerkang 624011,China)

机构地区：[1]四川省农业科学院植物保护研究所,农业部西南作物有害生物综合治理重点实验室,成都610066 [2]阿坝藏族羌族自治州农业科学技术研究所,马尔康624000 [3]阿坝州生态保护与发展研究院,汶川623000 [4]阿坝州农产品质量检验监测中心,马尔康624011 [5]阿坝州植保植检站,马尔康624011

出　　处：《应用昆虫学报》2024年第5期1081-1090,共10页Chinese Journal of Applied Entomology

基　　金：四川省阿坝州应用技术研究与开发资金项目(R22YYJSYJ0010);2023年四川省“三区”人才支持计划。

摘　　要：【目的】明确蓼蓝齿胫叶甲Gastrophysa atrocyanea取食酸模Rumex acetosa和唐古特大黄Rheum tanguticum后肠道微生物多样性的特点。【方法】通过高通量测序技术,分析取食酸模和唐古特大黄的蓼蓝齿胫叶甲体内肠道微生物种类、丰度、优势度、多样性以及功能变化。【结果】蓼蓝齿胫叶甲取食酸模和唐古特大黄后肠道微生物共有154种,其中取食酸模的独有种548种,取食唐古特大黄的独有种554种;变形菌门Proteobacteria是这两组蓼蓝齿胫叶甲肠道微生物的优势群;取食唐古特大黄的第3个样本肠道微生物物种多样性最高;代谢功能预测物种组成结果表明,除沙雷氏菌Serratia,取食酸模的优势群为泛菌Pantoea,取食唐古特大黄的优势群为肠杆菌科Enterobacteriaceae。【结论】本研究初步明确了蓼蓝齿胫叶甲取食酸模和唐古特大黄后的肠道微生物多样性特点,为其寄主范围和种群暴发预测提供了一定的理论依据。[Aim]Gastrophysa atrocyanea is an insect that feeds on weeds of the family Polygonaceae.[Methods]Highthroughput sequencing analysis was used to investigate the type,abundance,dominance and diversity of,and functional changes in,the gut microbiota in G.atrocyanea that fed on either Rumex acetosa or Rheum tanguticum.[Results]A total of 154 gut microbiota species were identified,of which 548 were unique to G.atrocyanea that fed on R.acetosa and 554 to those that fed on R.tanguticum.The Proteobacteria was the dominant gut microbiota in both treatment groups.Species diversity was highest in G.atrocyanea that had fed on R.tanguticum.The species composition predicted from the results of a metabolic function analysis indicate that,except for Serratia,the dominant group in the R.acetosa treatment group was Pantobacterium,and the dominant group in the R.tanguticum treatment group was Enterobacterium.[Conclusion]This study preliminarily clarified the diversity characteristics of gut microbiota of G.atrocyanea that feed on R.acetosa and R.tanguticum,providing a theoretical basis for its host range and population outbreak prediction.

关 键 词：蓼蓝齿胫叶甲 酸模 唐古特大黄 肠道微生物 天敌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]