121份澳大利亚小麦材料抗条锈病鉴定及分子检测  

Identification and molecular detection of resistance to wheat stripe rust in 121 Australian wheat varieties

作　　者：黄洁 杨金叶[1] Davinder Singh ZHANG Peng Robert FPark 王保通[1] 李强[1,2] HUANG Jie;YANG Jinye;Davinder Singh;ZHANG Peng;Robert FPark;WANG Baotong;LI Qiang(State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,College of Plant Protection,Northwest A&F University,Yangling 712100,China;Plant Breeding Institute Cobbitty,School of Life and Environmental Sciences,The University of Sydney,2570 Narellan,NSW,Australia)

机构地区：[1]西北农林科技大学植物保护学院,作物抗逆与高效生产全国重点实验室,杨凌712100 [2]Plant Breeding Institute Cobbitty,School of Life and Environmental Sciences,The University of Sydney,2570 Narellan,NSW,Australia

出　　处：《植物病理学报》2024年第6期1158-1166,共9页Acta Phytopathologica Sinica

基　　金：国家重点研发计划(2021YFD1401001、2016YFD300705)。

摘　　要：由小麦条锈菌(Puccinia striiformisf. sp.tritici,Pst)引起的条锈病是世界范围内小麦上最重要的病害之一,培育和种植抗病品种是防治条锈病最经济有效的措施。为明确澳大利亚小麦材料的抗条锈病水平及所含抗条锈病基因,本研究利用8个澳大利亚Pst流行小种对121份小麦材料进行了苗期抗性鉴定,同时应用小麦抗条锈病基因Yr5、Yr9(1B/1R)、Yr10、Yr15、Yr17及Yr26的特异性分子标记进行了分子检测。结果表明,121份小麦材料中,21份(17.35%)对所有供试Pst小种均表现抗病,7份(5.79%)均表现感病,其他材料对一个或多个Pst小种表现抗病。分子检测结合抗病性鉴定结果显示,3份(2.48%)可能含有Yr5基因,15份(12.40%)可能含有Yr9,9份(7.44%%)可能含有Yr10, 6份(4.96%)可能含有Yr17,19份(15.70%)可能含有Yr26,没有检测到Yr15基因。此外,其中13份材料同时检测到两个或两个以上的抗条锈病基因,还有19份材料可能含有其他已知或未知的抗条锈基因。本研究为121份澳大利亚小麦材料的合理利用和小麦抗病育种提供了科学依据。Stripe rust,caused by Puccinia striformis f.sp.tritici(Pst),is one of the most important diseases of wheat worldwide.Breeding and cultivating disease-resistant varieties is the most economical and effective strategy to control stripe rust.Eight Australian races of Pst were used to evaluate the seedling resistance of 121 wheat varieties under controlled greenhouse conditions.The presence of genes Yr5,Yr9,Yrl0,Yrl5,Yr17 and Yr26 was assessed by genotyping with linked molecular markers for each gene.A total of 21(17.35%)of 121 wheat varieties were resistant to all tested Pst races,seven(5.79%)varieties were susceptible to all Pst races.Based on molecular detection and disease resistance identification results,3(2.48%)wheat varieties may contain Yr5 gene,15(12.40%)may contain Yr9,9(7.44%)may contain Yr10,6(4.96%)may contain Yr17,19(15.70%)may contain Yr26 and no varieties were detected to carry Yr15.Two or more stripe rust resistance genes in combination were detected in 13 wheat varieties.In addition,19 varieties may contain other known or unknown new stripe rust resistance genes,which need further studies.This study provided scientific basis for rational utilization of 121 wheat varieties and breeding of wheat disease resistance.

关 键 词：小麦 条锈病 小麦条锈菌 抗性鉴定 抗病基因 分子检测 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]