滇黄精叶斑病致病菌的分离鉴定  

Isolation, identification of pathogen causing leaf spot on Polygonatum kingianum

作　　者：徐荣 王思婷 王占娣 杨世韦 刘科新 徐永艳[2] 焦蓉 XU Rong;WANG Siting;WANG Zhandi;YANG Shiwei;LIU Kexin;XU Yongyan;JIAO Rong(Yuxi Normal University,Yuxi 653100,China;Southwest Forestry University,Kunming 650224,China;Yunnan Nanbao Biotechnology Co.,Ltd,Yuxi 653100,China)

机构地区：[1]玉溪师范学院,玉溪653100 [2]西南林业大学,昆明650224 [3]云南南宝生物科技有限责任公司,玉溪653100

出　　处：《植物病理学报》2024年第6期1252-1256,共5页Acta Phytopathologica Sinica

基　　金：云南省地方本科高校基础研究联合专项(202101BA070001-055);云南省高校玉溪特色农业资源发掘与利用重点实验室。

摘　　要：0 引言滇黄精(Polygonatum kingianum Coll. et Hemsl),是百合科(Liliaceae)黄精属(Polygonatum)多年生草本植物,为云南省道地药材。黄精多糖是其中最重要的活性成分,药食同源,极具开发价值[1-3]。2022年6月在云南省新平县建兴镇(23.72°N,101.58°E)滇黄精种植基地中,发现一种病害,此病害在新平种植基地呈连续发生态势,田间发病率在25%~30%,7~9月高温多雨,通风不畅,肥水不当的地块发病率高达45%。A leaf spot of Polygonatum kingianum was found in Jianxing Town,Xinping County,Yunnan Province.Leaf infections first appeared as small,pale brown spots with a yellow haloes.The spots extended or coalesced into larger lesions,causing the entire leaf to be necrotic.The pathogen causing this disease was isola-ted,and its pathogenicity was tested according to the Koch's postulates.Based on morphological characteristics and phylogenetic analysis of combined the rDNA 18S and ITS regions as well as beta-tubulin(TUB)gene sequences,the isolate DYB-2 was identified as Neopestalotiopsis acrostichi.To our knowledge,this is the first report of N.acrostichi causing the leaf spot on Polygonatum kingianum in the world.

关 键 词：田间发病率 多年生草本植物 种植基地 道地药材 滇黄精 发生态势 药食同源 黄精属 

分 类 号：S432.44[农业科学—植物病理学]