牛膝茎基腐病的病原菌分离与鉴定  

Isolation and identification of the pathogen causing basal stem rot of Achyranthes bidentata

作　　者：王飞[1] 杨瑾 李绍建[1] 高素霞[1] 李雪梦 秦艳红[1] 赵莹 鲁传涛[1] WANG Fei;YANG Jin;LI Shaojian;GAO Suxia;LI Xuemeng;QIN Yanhong;ZHAO Ying;LU Chuantao(Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]河南农业大学植物保护学院,郑州450002

出　　处：《植物病理学报》2024年第6期1268-1272,共5页Acta Phytopathologica Sinica

基　　金：国家中药材产业技术体系(CARS-21);河南省中药材产业技术体系(HARS-22-11-Z1);河南省农科院新兴学科(2022XK07、2023XK05)。

摘　　要：0 引言牛膝(Achyranthes bidentata Blume.)为苋科多年生草本植物,又称山苋菜、对节菜、牛茎等,以干燥根入药,具有逐瘀通经、补肝肾、强筋骨、引血下行的功效[1],在我国分布广泛,药用历史悠久[2]。近年来,随着栽培面积的不断扩大,牛膝病害发生日益严重,已报道的牛膝主要病害有叶斑病、枯萎病、根腐病和根结线虫病等[3-4],对牛膝的产量与品质均造成极大影响。From 2020 to 2022,a new disease,basal stem rot of Achyranthes bidentata was prevalent in the field in Wuzhi county,Jiaozuo city,Henan province.The incidence of severe disease plot was over 90%,that seri-ously affected the yield and quality of A.bidentata.Tissue isolation and culture method was used to isolate and purify the pathogen from disease samples of A.bidentata with the typical symptoms of stem rot.Based on the morphological identification,sequence analysis of ITS and TEF-lα,and pathogenicity assays,the pathogen were identified as Rhizoctonia solani,and belonged to anastomosis subgroup(AG)-4-HGI.This is the first re-port of R.solani AG-4-HGI causing basal stem rot on A.bidentata in China,the results of this study could pro-vide useful guidance for the effective prevention and control of A.bidentatae basal stem rot.

关 键 词：根结线虫病 茎基腐病 多年生草本植物 病害发生 枯萎病 牛膝 根腐病 栽培面积 

分 类 号：S432.1[农业科学—植物病理学]