海鲜菇采后贮藏过程的转录组分析及褐变基因的挖掘  

Transcriptome Analysis of Hypsizygus marmoreus of Post Harvest Storage Process and Exploration of Browning Genes

作　　者：王伟霞 童小嫣 陈源 韩琦 赵书光[3] 李福后 WANG Weixia;TONG Xiaoyan;CHEN Yuan;HAN Qi;ZHAO Shuguang;LI Fuhou(Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University/Jiangsu Key Laboratory of Marine Biotechnology,Lianyungang,Jiangsu 222005;Co-Innovation Center of Jiangsu Marine Bio-Industry Technology,Lianyungang,Jiangsu 222000;Agricultural Technology Extension Center of Jiangsu Guannan County,Guannan,Jiangsu 222500)

机构地区：[1]江苏海洋大学海洋资源与环境重点实验室,海洋生物技术重点实验室,江苏连云港222005 [2]江苏省海洋生物产业技术协同创新中心,江苏连云港222000 [3]江苏省灌南县农业技术推广中心,江苏灌南222500

出　　处：《北方园艺》2025年第1期21-28,共8页Northern Horticulture

基　　金：江苏海洋大学2023年大学生创新创业训练计划资助项目(SY202311641638004)。

摘　　要：以海鲜菇子实体为试材,采用高通量测序技术,研究了海鲜菇子实体在贮藏过程中的转录组变化,以期为揭示子实体采后品质变化、抑制子实体褐变发生提供参考依据。结果表明:在4℃贮藏条件下,第10天与第0天相比,获得3659个差异表达基因,其中上调基因1707个,下调基因1952个;1-MCP处理后第10天与第0天相比,获得4142个差异表达基因,其中上调基因2122个,下调基因2020个。共筛选得到3个差异表达的漆酶基因和1个差异表达的过氧化物酶基因,分别是LAC1、LAC2、LAC4和POD2,这些基因可能与子实体褐变发生具有密切联系。Taking the fruit body of Hypsizygus marmoreus as the test meterial,the transcriptome changes of post harvest Hypsizygus marmoreus fruiting bodies during storage were studied by using high-throughput sequencing technology,in order to provide reference for revealing post harvest quality changes and inhibiting fruiting body browning.The results showed that 3659 differentially expressed genes were obtained on the 10th day compared to the 0th day,included 1707 upregulated genes and 1952 downregulated genes under storage conditions of 4℃.Moreover,on the 10th day after 1-MCP treatment,compared to the 0th day,4142 differentially expressed genes were obtained,included 2122 upregulated genes and 2020 downregulated genes.Three differentially expressed laccase genes and one differentially expressed peroxidase gene were screened,namely LAC1,LAC2,LAC4,and POD2.These genes might be closely related to the occurrence of browning in fruiting bodies.

关 键 词：海鲜菇 转录组测序 漆酶 过氧化物酶 

分 类 号：S759.81[农业科学—森林经理学]