Ⅲ型纤维素高固酶解及纤维素酶动态吸附过程研究  

Studies on enzymatic hydrolysis of celluloseⅢand dynamic cellulase adsorption at high solid loading

作　　者：李晨 翟睿 蒋笑笑 李海祥 金明杰 LI Chen;ZHAI Rui;JIANG Xiaoxiao;LI Haixiang;JIN Mingjie(School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing 210094,China)

机构地区：[1]南京理工大学环境与生物工程学院,江苏南京210094

出　　处：《生物加工过程》2025年第1期23-31,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金面上项目(22078160)。

摘　　要：高效高固含量酶解过程是木质纤维素生物炼制大规模工业化的前提。然而,木质纤维素酶解转化率随着固含量增加而下降,导致高固含量酶解成为生物炼制的一大难题。本研究对比了Ⅲ型纤维素及Ⅰ型纤维素高固酶解时的酶解效率及纤维素酶动态吸附过程,探究了不同抑制物对酶解的影响,并考察了外切葡聚糖酶分子结构对纤维素酶解效率的影响。结果表明,与Ⅰ型纤维素相比,Ⅲ型纤维素在高固酶解及特定抑制物存在情况下均具有优势性,其中外切葡聚糖酶的碳水化合物结合模块对Ⅲ型纤维素酶解优势有显著影响。该研究结果为解决木质纤维素高固酶解转化率低的难题提供了新思路。Efficient enzymatic hydrolysis at high solid loading is crucial for the largescale industrialization of lignocellulosic biorefinery.At present,one major challenge is that cellulose conversion often drops when solid content increases in the hydrolysis system.Herein,we first compared the enzymatic hydrolysis and adsorption of cellulases on celluloseⅢand celluloseⅠ,then explored various degradation products from enzymatic hydrolysis,and studied the important role of cellobiohydrolase on hydrolysis performance of celluloseⅢand celluloseⅠat molecular structure level.As a result,the hydrolysis of celluloseⅢat high solid loading and in the presence of various inhibitors proceeded much better than celluloseⅠ.In addition,the carbohydratebinding module(CBM)in cellobiohydrolase played an essential role in the preferred hydrolysis of celluloseⅢ.The findings in our study offer a new perspective for overcoming the challenges associated with low conversion of cellulose,especially at high solid loading.

关 键 词：高固酶解 Ⅲ型纤维素 抑制物 动态吸附 木质纤维素 生物炼制 

分 类 号：Q556[生物学—生物化学] TQ352[化学工程]