高效液相色谱法测定化妆品中艾地苯醌和辅酶Q_(10)  

Determination of idebenone and coenzyme Q_(10)in cosmetics by high performance liquid chromatography

作　　者：王继双 李莉[1] 张隆龙 王海燕[1] WANG Jishuang;LI Li;ZHANG Longlong;WANG Haiyan(National Institutes for Food and Drug Control,NMPA Key Laboratory for Researching and Evaluation of Cosmetics,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,国家药品监督管理局化妆品研究与评价重点实验室,北京100050

出　　处：《化学分析计量》2025年第1期6-11,共6页Chemical Analysis And Meterage

基　　金：国家科技重大专项(2022FY101202)。

摘　　要：建立高效液相色谱法测定化妆品中艾地苯醌和辅酶Q_(10)的含量。样品经无水乙醇超声提取,用Waters XBridge C_(18)色谱柱(250 mm×4.6 mm,5μm)分离。以无水乙醇-水作为流动相进行梯度洗脱,流量为1.0 mL/min,柱温为40℃,检测波长为275 nm,以色谱峰面积外标法定量。艾地苯醌和辅酶Q_(10)的质量浓度在0.5~100 mg/L范围内与色谱峰面积具有良好的线性关系,线性相关系数均为0.9999。艾地苯醌和辅酶Q_(10)的方法检出限分别为0.11、0.41 mg/kg,定量限分别为0.37、1.35 mg/kg。爽肤水、乳液、面霜和凝胶4种基质3个浓度水平的加标平均回收率为94.7%~102.6%,测定结果的相对标准偏差为0.3%~2.8%(n=6)。该方法操作简便,适用于化妆品中艾地苯醌和辅酶Q_(10)的测定。A method for determining the content of idebenone and coenzyme Q_(10)in cosmetics by high performance liquid chromatography was established.The samples were ultrasonically extracted with anhydrous ethanol and separated using a Waters XBridge C_(18)column(250 mm×4.6 mm,5μm).Gradient elution was performed with anhydrous ethanolwater as the mobile phase at a flow rate of 1.0 mL/min,with column temperature of 40℃ and detection wavelength of 275 nm.Quantitative analysis was conducted using the external standard method based on chromatographic peak areas.The mass concentrations of idebenone and coenzyme Q_(10)had a good linear relationship with the chromatographic peak area in the range of 0.5-100 mg/L,the correlation coefficients were all 0.9999.The detection limits of idebenone and coenzyme Q_(10)was 0.11,0.41 mg/kg,respectively,and the quantification limits was 0.37,1.35 mg/kg,respectively.The average recoveries of the four matrices(toner,lotion,cream and gel)at three concentration levels were 94.7%-102.6%,and the relative standard deviations of the determination results was 0.3%-2.8%(n=6).This method is simple and convenient to operate,making it suitable for the determination of idebenone and coenzyme Q_(10)in cosmetics.

关 键 词：化妆品 高效液相色谱法 艾地苯醌 辅酶Q_(10) 

分 类 号：O657.7[理学—分析化学]