高效液相色谱法同时测定化妆品中25种美白成分  

Simultaneous determination of 25 whitening ingredients in cosmetics by high performance liquid chromatography

作　　者：吴姣娇 赵薇 潘小红 涂晓琴 谭雪莹 殷帅 陈真 WU Jiaojiao;ZHAO Wei;PAN Xiaohong;TU Xiaoqin;TAN Xueying;YIN Shuai;CHEN Zhen(Hunan Institute for Drug Control,Hunan Engineering&Technology Research Center for Pharmaceutical Quality Evaluation,Changsha 410000,China;Hunan Drug Inspection Center,Changsha 410000,China)

机构地区：[1]湖南省药品检验检测研究院,湖南省药品质量评价工程技术研究中心,长沙410000 [2]湖南省药品审核查验中心,长沙410000

出　　处：《化学分析计量》2025年第1期26-33,共8页Chemical Analysis And Meterage

基　　金：湖南省自然科学基金-部门联合基金资助项目(2023JJ60108)。

摘　　要：建立高效液相色谱法同时测定化妆品中25种美白成分。化妆品样品经体积分数为50%的甲醇溶液超声提取,用聚四氟乙烯滤膜过滤,Thermo Scientific AcclaimTM 120(250 mm×4.6 mm,5μm)色谱柱梯度洗脱分离,二极管阵列检测器检测,外标法定量。25种美白成分的质量浓度在0.4~392.5μg/mL范围内与色谱峰面积线性关系良好,线性相关系数均大于0.999,在水基、乳剂、粉剂、面膜、凝胶5种基质中的检出限为0.4~41.4μg/g,定量限为1.3~138.1μg/g。样品加标平均回收率为92.6%~109.7%,测定结果的相对标准偏差为0.3%~4.8%(n=6)。该方法可用于多种化妆品基质中25种美白成分的测定。A method for simultaneous determination of 25 whitening components in cosmetics by high performance liquid chromatography was established.Cosmetic samples were extracted by 50%(volume fraction)methanol solution ultrasound,filtered by polytetrafluoroethylene(PTFE)membrane,separated by gradient elution on Thermo Scientific AcclaimTM 120(250 mm×4.6 mm,5μm)chromatography column,detected by diode array detector(DAD),and quantified by external standard method.The mass concentration of 25 whitening components had a good linear relationship with the chromatographic peak area in the range of 0.4-392.5μg/mL,and the linear correlation coefficients were all more than 0.999.The detection limits were 0.4-41.4μg/g and the quantification limits were 1.3-138.1μg/g in water base,emulsion,powder,facial mask and gel.The average recoveries of spiked samples were 92.6%-109.7%,and the relative standard deviations of determination results were 0.3%-4.8%(n=6).This method can be used for the determination of 25 whitening components in most cosmetic matrices.

关 键 词：化妆品 祛斑 美白 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]