高效液相色谱内标法测定毒品中氟胺酮  

Determination of F-Ketamine in drugs by high performance liquid chromatography with internal standard method

作　　者：梁舒婷 蒙卫宁 吴明霞 廖雪晴 何锡超 陈乐 LIANG Shuting;MENG Weining;WU Mingxia;LIAO Xueqing;HE Xichao;CHEN Le(Drug Intelligence and Technology Center of the Public Security Department of Guangxi,Nanning 530000,China;Guangxi Police College,Nanning 530000,China)

机构地区：[1]广西壮族自治区公安厅禁毒情报技术中心,南宁530000 [2]广西警察学院,南宁530000

出　　处：《化学分析计量》2025年第1期54-57,共4页Chemical Analysis And Meterage

基　　金：广西高校中青年教师科研基础能力提升项目(2023KY0909)。

摘　　要：建立测定毒品中氟胺酮含量的高效液相色谱内标法。样品经称量、溶解、摇匀和过滤后,经Waters symmetry■C_(18)(250 mm×4.6 mm,5μm)色谱柱分析,以磷酸-三乙胺溶液和甲醇作为流动相进行等度洗脱(体积比为70∶30),检测波长为210 nm。氟胺酮的质量浓度在2~500 mg/L范围内与色谱峰面积线性关系良好,相关系数为0.9999,检出限为1 mg/L,定量限为2 mg/L。低、中、高3个浓度水平的加标平均回收率为99.0%~102.2%,测定结果的相对标准偏差为0.71%~4.05%(n=6)。该方法分离效果和稳定性好,适用于固体毒品中氟胺酮的定量分析。A high performance liquid chromatography internal standard method for determining the content of FKetamine(2F-DCK)in drugs was established.The sample was weighed,dissolved,shaken evenly,and filtered,then analyzed on a Waters symmetry■C_(18)(250 mm×4.6 mm,5μm)chromatographic column,with phosphate acid-triethylamine solution and methanol as the mobile phase for isocratic elutionthe(the volume was 70∶30),and the detection wavelength was 210 nm.The mass concentration of F-Ketamine had a good linear relationship with chromatographic peak area in the range of 2-500 mg/L,with a correlation coefficient of 0.9999.The detection limit of the method was 1 mg/L,and the quantification limit was 2 mg/L.The recoveries of low,medium,and high levels of spiked samples were 99.0%-102.2%,and the relative standard deviations of the measurement results were 0.71%-4.05%(n=6).The method has good separation effect and stability,which is suitable for quantitative analysis of F-Ketamine in solid drugs.

关 键 词：氟胺酮 高效液相色谱法 内标法 毒品 

分 类 号：O657.7[理学—分析化学]