液相色谱-质谱法测定淡水鲈鱼中3-氨基-2-唑酮的不确定度评定  

Evaluation of uncertainty for determination of 3-amino-2-oxazolone in freshwater perch by liquid chromatography-mass spectrometry

作　　者：黄海霞 廖子祎 王海波 李锐 陆柔 杨尚超 HUANG Haixia;LIAO Ziyi;WANG Haibo;LI Rui;LU rou;YANG Shangchao(Guangxi-ASEAN Food Inspection and Testing Center,Nanning 530028,China)

机构地区：[1]广西-东盟食品检验检测中心,南宁530028

出　　处：《化学分析计量》2025年第1期129-134,共6页Chemical Analysis And Meterage

基　　金：广西重点研发计划(桂科AB24010013)。

摘　　要：利用液相色谱-质谱法测定淡水鲈鱼中呋喃唑酮代谢物3-氨基-2-唑酮(AOZ),并对测定结果的不确定度进行评定,分析影响测定结果准确性的因素,从而提高检测结果准确性。按照GB 31656.13—2021《食品安全国家标准水产品中硝基呋喃类代谢物多残留的测定液相色谱-串联质谱法》测定淡水鲈鱼中的AOZ,建立数学模型,对影响测定结果的不确定度来源如标准物质纯度、溶液配制、样品称量、样品溶液制备、标准曲线拟合、测量复性等因素进行分析和评定。液相色谱-质谱法测定淡水鲈鱼中AOZ的结果为(10.09±1.10)μg/kg,k=2。标准物质纯度、溶液配制、标准曲线拟合以及样品测量重复性是测量不确定度的主要来源,在检测过程中应加以控制。Liquid chromatography-mass spectrometry was used to determine the metabolite 3-amino-2-oxazolone(AOZ)of furazolidone in freshwater perch,and the uncertainty of the determination results was evaluated,and the key factors affecting the accuracy of the detection results were analyzed to improve the accuracy of the detection results.According to GB 31656.13—2021"National food safety standard—Determination of nitrofuran metabolites residues in aquatic products by liquid chromatography-tandem mass spectrometry method",the AOZ in freshwater perch was determined.Established a mathematical model to analyze and evaluate the sources of uncertainty that affected the measurement results,such as purity of the standard material,solution preparation,sample weighing,sample solution preparation,standard curve fitting,and measurement repeatability of sample.The determination results of AOZ in freshwater perch by liquid chromatography-mass spectrometry was(10.09±1.10)μg/kg,k=2.The purity of standard substances,solution preparation,standard curve fitting,and measurement repeatability of sample were the main sources of measurement uncertainty,which should be controlled during the detection process.

关 键 词：液相色谱-质谱法 淡水鲈鱼 3-氨基-2-唑酮 呋喃唑酮代谢物 不确定度 

分 类 号：O657.7[理学—分析化学]