基于多指标-响应面法优选甘草制巴戟天工艺及其总寡糖体外抗氧化活性研究  

Optimal processing technology of Glycyrrhizae Radix et Rhizoma processed Morindae Officinalis Radix with multi-indicator-response surface methodology and antioxidant effect evaluation

作　　者：刘梦云 闫小景 王宸 朱丽君 朱建光[1,2,3] 康乐 田连起 魏玉[1] 李红伟[1,2,3] 李凯[1,2,3] LIU Mengyun;YAN Xiaojing;WANG Chen;ZHU Lijun;ZHU Jianguang;KANG Le;TIAN Lianqi;WEI Yu;LI Hongwei;LI Kai(Henan University of Traditional Chinese Medicine,Henan Province,Zhengzhou 450046,China;Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao,Henan Province,Zhengzhou 450046,China;Henan Research Center for Special Processing Technology of Chinese Medicine,Henan Province,Zhengzhou 450046,China;Luohe Second People’s Hospital,Henan Province,Luohe 462000,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]豫药全产业链研发河南省协同创新中心,河南郑州450046 [3]河南省中药特色炮制技术工程研究中心,河南郑州450046 [4]漯河市第二人民医院,河南漯河462000

出　　处：《中草药》2024年第23期7989-7998,共10页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年项目(82404865);河南省重点研发与推广专项(科技攻关)项目(242102310542)。

摘　　要：目的优选甘草制巴戟天Morindae Officinalis Radix Praeparata的最佳炮制工艺,并考察其炮制前、后总寡糖对雄性小鼠睾丸支持细胞(TM4)氧化损伤的保护作用。方法采用多指标-响应面法,以D-果糖、D(+)-无水葡萄糖、蔗糖、蔗果三糖、耐斯糖、1^(F)-果呋喃糖基耐斯糖共6种糖类成分的含量为评价指标,利用层次分析法(analytical hierarchy process,AHP)-熵权法计算各评价指标的权重系数和综合评分;进一步利用Box-Behnken设计-响应面法(Box-Behnken design-response surface method,BBD-RSM)考察甘草用量、闷润时间、煎煮时间对甘草制巴戟天炮制工艺的影响,优选最佳炮制工艺;按照最佳炮制工艺制备甘草制巴戟天,并评估甘草制前、后巴戟天总寡糖对H_(2)O_(2)诱导的TM4细胞氧化应激损伤的保护作用。结果甘草制巴戟天的最佳炮制工艺为100 g生巴戟天饮片,甘草用量8 g,闷润时间1.0 h、煎煮时间35 min。药效实验结果表明,甘草制巴戟天总寡糖可显著增强谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)和超氧化物歧化酶(superoxide dismutase,SOD)活性(P<0.01)、减少丙二醛(malonaldehyde,MDA)含量(P<0.05),且抗氧化应激损伤作用优于生品总寡糖。结论运用AHP-熵权法结合BBD-RSM优选制巴戟天炮制工艺,结果准确、可行;药效实验结果为巴戟天甘草制的合理性提供实验依据。Objective To optimize the processing technology of Gancao(Glycyrrhizae Radix et Rhizoma,GRR)processed Bajitian(Morindae Officinalis Radix,MOR)and compare the effects of MOR oligosaccharides on the oxidative damage of testicular support(TM4)cells in male mice before and after processing.Methods A multi-index-response surface methodology was used,with D-fructose,D(+)-glucose,sucrose,kestose,nystose,and 1^(F)-fructofuranosylnystose as evaluation indicators.Analytical hierarchy process(AHP)-entropy weighting method was used to calculate the weight of each indicator and determine the comprehensive score(OD).Furthermore,Box-Behnken design-response surface methodology(BBD-RSM)was used to investigate the effects of GRR dosage ratio,moistening time and decoction time on the processing technology of MOR.The processed MOR was prepared according to the optimal processing technology to evaluate the protective effect of MOR oligosaccharides on oxidative stress damage induced by hydrogen peroxide in male mice testicular support cells.Results The best processing parameters were 100 grams raw MOR and 8 g GRR dosage for moistening for 1 h,decocting time was 35 min.The results of pharmacodynamic experiments demonstrated that GRR processed MOR oligosaccharides had significantly enhanced GSH-Px,CAT and SOD enzyme activities,reduced MDA contents(P<0.05),and had stronger antioxidative effects than the raw MOR oligosaccharides.Conclusion In this experiment,the AHP-entropy weighting method combined with BBD-RSM was used to optimize the process parameters of GRR processed MOR,which was accurate and feasible.And the results of pharmacodynamic experiments could provide experimental basis for the rationality of GRR processed MOR.

关 键 词：制巴戟天 炮制工艺 寡糖 响应曲面法 氧化损伤 层次分析法 熵权法 D-果糖 D(+)-无水葡萄糖 蔗糖 蔗果三糖 耐斯糖 1F-果呋喃糖基耐斯糖 Box-Behnken设计-响应面法 谷胱甘肽过氧化物酶 过氧化氢酶 超氧化物歧化酶 丙二醛 

分 类 号：R283.6[医药卫生—中药学]