基于“开窍通络”探究艾片改善冠脉微循环障碍的作用机制  

Mechanism of Blumea balsamifera in improving coronary microcirculatory disorders based on“Kaiqiao Tongluo”

作　　者：侯薇 陈兰英[1,2] 袁蓓欣 崔亚茹[1,2] 段文彬 汪文龙 刘荣华[1] HOU Wei;CHEN Lanying;YUAN Beixin;CUI Yaru;DUAN Wenbin;WANG Wenlong;LIU Ronghua(Jiangxi University of Chinese Medicine,Nanchang 330004,China;National Pharmaceutical Engineering Research Center of Solid Preparation in Chinese Herbal Medicine,Nanchang 330006,China6)

机构地区：[1]江西中医药大学,江西南昌330004 [2]中药固体制剂制造技术国家工程研究中心,江西南昌3300065

出　　处：《中草药》2024年第23期8079-8090,共12页Chinese Traditional and Herbal Drugs

基　　金：江西省自然科学基金重点项目(20224ACB206043);国家自然科学基金项目(82160732);国家重点研发计划项目(2018YFC1706102);中药药效物质基础江西省重点实验室项目(2024SSY07102)。

摘　　要：目的探究艾片基于“开窍通络”改善冠脉微循环障碍(coronary microcirculation disorders,CMD)大鼠的作用及可能机制。方法选取雄性SD大鼠48只,随机分为假手术组、模型组和艾片低、高剂量组(0.05、0.10 g/kg),除假手术组注射等量PBS外,其余组大鼠经左心室注射聚乙烯微球混悬液构建CMD大鼠模型。观察中医证候宏观评价指标舌象;检测血流动力学中CMD大鼠血流量(mean blood flow,MBF)、动脉平均压(mean arterial pressure,MAP)及左心室压变化;检测血液流变学中血液黏度、凝血四项、血小板功能指标的异常变化情况;生化分析仪及试剂盒检测大鼠血清中心功能、内皮功能及能量代谢相关指标含量变化;苏木素-伊红(hematoxylin-eosin,HE)染色观察心肌组织病变情况;通过可视化技术结合Angio Tool软件及泊肃叶公式分析冠脉微血管面积、密度、直径、空隙度及血流阻力;Western blotting检测心肌组织中能量代谢相关蛋白肝激酶B1(liver kinase B1,LKB1)、腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)、磷酸化腺苷酸活化蛋白激酶(phospho-AMPK,p-AMPK)、过氧化物酶体增殖物激活受体γ辅激活酶因子-1α(peroxisome proliferators-activated receptorγcoactivator-1 alpha,PGC-1α)及血管新生相关蛋白血管内皮生长因子(vascular endothelial growth factor,VEGF)、血管内皮细胞生长因子受体2(vascular endothelial growth factor receptor 2,VEGFR2)蛋白表达情况。结果与假手术组比较,艾片可显著升高CMD大鼠MBF、MAP(P<0.01、0.05),改善左心室压异常现象;显著降低CMD大鼠平均血小板体积(mean platelet volume,MPV),升高血小板计数(platelet count,PLT),显著延长凝血酶原时间(prothrombin time,PT)及活化部分凝血酶原时间(activated partial thromboplastin time,APTT),改善CMD大鼠血液流变学异常情况(P<0.05、0.01);显著降低血清中肌酸激酶(creatine kinase,CK)、肌酸激酶同工酶(creatine kinase MB,CK-MB)、Objective To investigate the effects and possible mechanisms of Blumea balsamifera in improving coronary microcirculation disorders(CMD)in rats based on“Kaiqiao Tongluo”.Methods A total of 48 male SD rats were selected and randomly divided into sham group,model group,low-and high-dose group of B.balsamifera(0.05,0.10 g/kg).Except for the sham group,which was injected with an equal amount of PBS,the other three groups were injected with polyethylene microsphere suspension into the left ventricle to prepare CMD rat models.Observing the tongue image,a macroscopic evaluation index of traditional Chinese medicine evidence,detecting the changes of mean blood flow(MBF),mean arterial pressure(MAP)and left ventricular pressure in CMD rats in hemodynamics,detecting the abnormal changes of blood viscosity,coagulation four items,and platelet function indexes in blood rheology,and the changes of the content of serum central function,endothelial function,and energy metabolism-related indexes were detected by biochemical analyzers and kits.Hematoxylin-eosin(HE)staining was used to observe myocardial tissue lesions,coronary microvessel area,density,diameter,voidness,and resistance to blood flow were analyzed by visualization technology combined with Angio Tool software and Poisson Leaf formula,and energy metabolism-related proteins liver kinase B1(LKB1),AMP-activated protein kinase(AMPK),phospho-AMPK(p-AMPK),peroxisome proliferators-activated receptorγcoactivator-1 alpha(PGC-1a),and vascular neovascularization-related proteins vascular endothelial growth factor(VEGF)and vascular endothelial growth factor receptor 2(VEGFR2)protein expression were detected byWestern blotting.Results Compared with the sham surgery group,B.balsamifera significantly increased MBF and MAP in CMD rats(P<0.05,0.01),and improved abnormal left ventricular pressure;significantly reduced mean platelet volume(MPV)and increased platelet count(PLT)in CMD rats,significantly prolonged prothrombin time(PT)and activated partial thromboplastin time(APTT),and i

关 键 词：艾片 冠脉微循环障碍 微血管可视化技术 能量代谢 内皮功能 促进血管新生 纠正血管舒缩障碍 开窍通络 

分 类 号：R285.5[医药卫生—中药学]