丙二酰基人参皂苷对南洋参和线叶南洋参细胞生长及皂苷积累的影响  

Effects of malonyl-ginsenosides on cell growth and saponin accumulation of Polyscias fruticosa and Polyscias filicifolia

作　　者：吕思琴 Nosov Aleander Mihaylovich Eugene A.Rogozhin Andrey A.Gontcharov 杨玲[1,6] LYU Siqin;Nosov Alexander Mihaylovich;Eugene A.Rogozhin;Andrey AGontcharov;YANG Ling(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;Department of Plant Physiology,Biological Faculty,Lomonosov Moscow State University,Moscow 119991,Russia;Department of Cell Biology,Institute of Plant Physiology K.A.Timiryazev,Russian Academy of Sciences,Moscow 127276,Russia;Plant Defense Peptide Laboratory,Institute of Bioorganic Chemistry,Russian Academy of Sciences,Moscow 117997,Russia;Federal Centre for Land Biodiversity,Far East Branch of the Russian Academy of Sciences,Vladivstok 690020,Russia;Shenzhen MSU-BIT University,Shenzhen 518172,China)

机构地区：[1]东北林业大学林学院,林木遗传育种全国重点实验室,黑龙江哈尔滨150040 [2]莫斯科国立罗门诺索夫大学生物系,莫斯科119991 [3]俄罗斯科学院植物生理研究所细胞生物学实验室,莫斯科127276 [4]俄罗斯科学院生物有机化学研究所植物防御肽实验室,莫斯科117997 [5]俄罗斯科学院远东分院联邦陆地生物多样性中心,海参崴690020 [6]深圳北理莫斯科大学生物系,植物生物学实验室,广东深圳518172

出　　处：《中草药》2024年第23期8171-8184,共14页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划(2023YFD2200103)。

摘　　要：目的以南洋参Polyscias fruticose和线叶南洋参P.filicifolia的悬浮培养细胞为研究对象,研究在细胞培养过程中,丙二酰基人参皂苷(malonyl ginsenoside,M-GS)对细胞生长及皂苷合成的影响,以促进南洋参悬浮培养细胞的皂苷合成和积累。方法以南洋参和线叶南洋参的细胞系(编号6a和VDK)为试验材料,在悬浮培养的第7天添加M-GS,分析添加M-GS对细胞生长以及细胞中多糖苷E、人参皂苷R0、多糖苷A和齐墩果酸皂苷A含量的影响。结果M-GS对南洋参和线叶南洋参的生长均具有抑制作用,使其不能长期生存。M-GS可使南洋参6a细胞系的细胞密度以及干质量峰值提前出现,使细胞活力提前开始下降,缩短了细胞的培养周期。而线叶南洋参VDK细胞系的细胞密度、鲜质量以及干质量的峰值延迟出现,延长了细胞的培养周期。M-GS对南洋参6a细胞系的抑制作用比线叶南洋参VDK更强。在培养末期南洋参6a细胞系的细胞密度、活力、鲜质量以及干质量分别降低了25.43%、22.23%、46.56%以及45.56%。M-GS对南洋参和线叶南洋参细胞中皂苷积累的影响不同。对于南洋参6a细胞系,M-GS促进细胞中多糖苷E、多糖苷A、齐墩果酸皂苷A的合成,且对多糖苷E的促进能力最强(提高了79.67%),其次为齐墩果酸皂苷A(提高了70.67%),最后为多糖苷A(提高了19.67%),同时抑制了人参皂苷R0的含量(降低了74.00%)。对于线叶南洋参VDK细胞系,M-GS只促进了细胞中多糖苷A的合成,使其含量增加了63.29%,却抑制了细胞中多糖苷E、多糖苷A、齐墩果酸皂苷A的合成,且对多糖苷A抑制能力最强(降低了63.29%),其次为多糖苷E(减低了27.59%),最后为齐墩果酸皂苷A(减低了8.33%)。结论添加M-GS可以促进南洋参和线叶南洋参悬浮培养细胞的部分种类皂苷积累,但不宜于细胞长期培养;且M-GS对不同物种细胞系细胞中不同皂苷的影响不同,对南洋参6a细胞系的促进能力高于�Objective With suspension cultured cells of Polyscias fruticosa and P.filicifolia as research objects,we explored the effects of malonyl ginsenosides(M-GS)on the cell growth and saponin synthesis during the cell culture to promote the synthesis and accumulation of saponin in suspension cultured cells of P.fruticosa.Methods Cell lines(6a and VDK)of P.fruticosa and P.filicifolia were used as experimental materials.M-GS was added on 7th day of the suspension culture to analyze the effects of M-GS addition on the cell growth and contents of polyscioside E,ginsenoside R0,polyscioside A and ladyginoside A saponins in cells.Results M-GS could inhibit the growth of P.fruticosa and P.filicifolia,preventing their long-term survival.M-GS brought forward the peak of cell density and dry weight of 6a cell line of P.fruticosa,causing the cell viability to start declining earlier,and shortening the cell culture cycle.While the peak values of cell density,fresh weight and dry weight of VDK cell line were delayed,which prolonged the cell culture cycle.The inhibitory effect of M-GS on 6a cell line was stronger than that of VDK.The cell density,vitality,fresh weight and dry weight of 6a cell line decreased by 25.43%,22.23%,46.56%and 45.56%,respectively.The effects of M-GS on the accumulation of saponins in the cells of P.fruticosa and P.filicifolia were different.For the cell line 6a of P.fruticosa,M-GS promoted the synthesis of Polyscioside E,Polyscioside A and Ladyginoside A saponins,and the promotion was the strongest for Polyscioside E saponins(increased by 79.67%),followed by ladyginoside A saponins(increased by 70.67%)and polyscioside A saponins(increased by 19.67%).However,the content of ginsenoside R0 saponins was inhibited(reduced by 74.00%).For the VDK cell line of Panax lineolata,M-GS only promoted the synthesis of Polyscioside A saponins,increasing its content by 63.29%,but inhibited the synthesis of polyscioside A,Polyscioside A and Ladyginoside A saponins,and had the strongest inhibition on polyscioside A saponins(dec

关 键 词：南洋参 线叶南洋参 细胞培养 丙二酰基人参皂苷 多糖苷E 人参皂苷R0 多糖苷A 齐墩果酸皂苷A 

分 类 号：R286.2[医药卫生—中药学]