隔药饼灸抑制子宫内膜异位症小鼠子宫内膜黏附重建的作用机制研究  

Mechanism of Medicinal Cake-separated Moxibustion in Inhibition of Ectopic Endometrium Adhesion and Reconstruction in Mice with Endometriosis

作　　者：郭慧文 周平生[2] 黄丽丹 王颖 刘莹 周志刚[1] GUO Huiwen;ZHOU Pingsheng;HUANG Lidan;WANG Ying;LIU Ying;ZHOU Zhigang(Acupuncture and Tuina College of Jiangxi University of Chinese Medicine,Nanchang 330004,China;International Education College of Jiangxi University of Chinese Medicine,Nanchang 330006,China;Graduate School of Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学针灸推拿学院,南昌330004 [2]江西中医药大学国际教育学院,南昌330006 [3]江西中医药大学研究生院,南昌330004

出　　处：《世界中医药》2024年第21期3305-3310,3317,共7页World Chinese Medicine

基　　金：国家自然科学基金地区项目(82160931);江西自然科学基金面上项目(20212BAB206011);江西省教育厅科技项目-科技计划项目(GJJ201209,GJJ190640)。

摘　　要：目的:观察隔药饼灸干预子宫内膜异位症(EMs)模型小鼠异位内膜组织生长,调控尿激酶型纤溶酶原激活物(uPA)、基质金属蛋白酶9(MMP 9)、E-钙黏蛋白(E-cadherin)、轴蛋白(Axin)、糖原合酶激酶3β(GSK3β)和β联蛋白(β-catenin)的表达,探索隔药饼灸抑制EMs异位内膜黏附重建机制。方法:42只雌性Balb/c小鼠按照随机区组分组法分为正常组、模型组、隔药饼灸组、Wnt激动剂组和Wnt抑制剂组,采用自体移植法建立EMs小鼠模型,术后2周各组连续治疗14 d。末次治疗结束后计算小鼠扭体次数,测量异位内膜组织大小,蛋白质免疫印迹法检测异位内膜组织中uPA、MMP-9、E-caderin、Axin、GSK3β和β-catenin蛋白表达情况。结果:与模型组和Wnt激动剂组比较,隔药饼灸组小鼠扭体反应数显著减少,且异位囊泡体积显著缩小(P<0.05)。蛋白质免疫印迹结果显示,隔药饼灸组的Axin、GSK3β、β-catenin、uPA、MMP-9的蛋白表达显著低于模型组和Wnt激动剂组(P<0.05),隔药饼灸组的E-adherin蛋白表达水平显著高于模型组和Wnt激动剂组(P<0.05)。结论:隔药饼灸可通过降低子宫内膜β-catenin、Axin、GSK3β、uPA、MMP 9的表达,提高E-cadherin的表达,介导异位内膜组织“黏附-侵袭-血管生成”发展机制,抑制其异位内膜的黏附、阻止基质和基底膜重建过程,从而减轻EMs小鼠痛经症状、抑制其异位内膜组织生长。Objective:To observe the effects of medicinal cake-separated moxibustion on the growth of ectopic endometrial tissue in a mouse model of endometriosis(EMs)and the expression of urokinase-type plasminogen activator(uPA),matrix metalloproteinase 9(MMP-9),E-cadherin,Axin,glycogen synthase kinase 3β(GSK3β),andβ-catenin,and to explore the mechanism of medicinal cake-separated moxibustion in inhibiting the adhesion and reconstruction of ectopic endometrial tissue in EMs.Methods:Forty-two female Balb/c mice were randomly divided into six groups:normal group,model group,medicinal cake-separated moxibustion group,Wnt agonist group,and Wnt inhibitor group.An autologous transplantation method was used to establish the EMs mouse model.Treatment was administered for 14 consecutive days,starting two weeks after surgery.At the end of the treatment period,the number of body twists was recorded,and the size of ectopic endometrial tissue was measured.Western blot was used to detect the expression of uPA,MMP-9,E-cadherin,Axin,GSK3β,andβ-catenin in ectopic endometrial tissue.Results:Compared with the model group and Wnt agonist group,the medicinal cake-separated moxibustion group showed a significant reduction in the number of body twists and a significant decrease in the size of ectopic vesicles(P<0.05).Western blot results indicated that the protein expression of Axin,GSK3β,β-catenin,uPA,and MMP-9 in the medicinal cake-separated moxibustion group was significantly lower than that in the model and Wnt agonist groups(P<0.05).Moreover,the protein expression of E-cadherin was significantly higher in the medicinal cake-separated moxibustion group than in the model and Wnt agonist groups(P<0.05).Conclusion:Medicinal cake-separated moxibustion may reduce the expression ofβ-catenin,Axin,GSK3β,uPA,and MMP-9,and increase the expression of E-cadherin in endometrial tissue.It mediates the“adhesion-invasion-angiogenesis”developmental mechanism of ectopic endometrial tissue,inhibits its adhesion,and prevents the reconstruction of t

关 键 词：隔药饼灸 子宫内膜异位症 子宫异位内膜 黏附重建 β-连环蛋白 基质金属蛋白酶9 尿激酶型纤溶酶原激活物 E-钙黏蛋白 

分 类 号：R245.8[医药卫生—针灸推拿学]