枸杞子在大鼠体内的代谢产物及代谢途径分析  

Analysis on Metabolites and Metabolic Pathways of Lycii Fructus in Rats

作　　者：宗政 胡扬[1] 徐蓓蕾[1] 辛国松 陈鹰翔 李文兰[1] ZONG Zheng;HU Yang;XU Beilei;XIN Guosong;CHEN Yingxiang;LI Wenlan(College of Pharmacy,Harbin University of Commerce,Harbin 150076)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《中国医药工业杂志》2024年第12期1659-1675,共17页Chinese Journal of Pharmaceuticals

基　　金：黑龙江省重点研发计划项目(2022ZX02C08);黑龙江省双一流学科协同创新成果项目(LJGXCG2023-023)。

摘　　要：运用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS/MS)研究枸杞子提取物在大鼠血清、尿液、粪便、胆汁中的原型成分及代谢产物,并明确其代谢过程。采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm);以乙腈为流动相A,0.1%甲酸水溶液为流动相B,梯度洗脱。柱温为30℃,流速为0.3 mL/min,进样量为3μL。结果显示,从各生物样品中共鉴定出46个化学成分,其中包括17个原型成分和29个代谢产物,其代谢途径包括Ⅰ相代谢反应(羟基化、去羟基化、去甲基化、水解、还原)和Ⅱ相代谢反应(甲基化、葡萄糖醛酸化、硫酸酯化、磺酸化)。该研究初步阐明了枸杞子在体内的代谢过程,为后续阐明其药效物质基础和开发利用奠定了基础。The ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)method was established to study the prototypical components and metabolites of Lycii Fructus extract in serum,urine,faeces,and bile of rats and to clarify their metabolic processes.The Waters ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7μm)was used,and the analysis was carried out in the gradient elution mode with acetonitrile as mobile phase A,and 0.1%formic acid as mobile phase B.The column temperature was 30℃,the flow rate was 0.3 mL/min,and the injection volume was 3μL.The results showed that a total of 46 chemical components were identified from various biological samples,including 17 prototypical components and 29 metabolites.Its metabolic pathways included phaseⅠmetabolic reactions(hydroxylation,dehydroxylation,demethylation,hydrolysis,and reduction)and phaseⅡmetabolic reactions(methylation,glucuronidation,sulfation,and sulfonation).This study preliminarily elucidated the metabolic process of Lycii Fructus in vivo,and laid a foundation for the subsequent elucidation of its pharmacological material basis and development.

关 键 词：枸杞子 UPLC-Q-TOF-MS/MS 代谢产物 代谢途径 

分 类 号：R917[医药卫生—药物分析学] R285.5[医药卫生—药学]