乌梅抗肝纤维化的作用及机制研究  

Effect and mechanism of Prunus mume against hepatic fibrosis

作　　者：郝峰 李冀[2] 杜菁 欧阳宇晨 崔艺春 魏爽 HAO Feng;LI Ji;DU Jing;OUYANG Yuchen;CUI Yichun;WEI Shuang(College of Clinical Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;College of Basic Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;College of Acupuncture,Moxibustion and Massage,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学临床医学院,南昌330004 [2]黑龙江中医药大学基础医学院,哈尔滨150040 [3]江西中医药大学中医学院,南昌330004 [4]江西中医药大学针灸推拿学院,南昌330004

出　　处：《中国药房》2025年第2期172-178,共7页China Pharmacy

基　　金：江西省卫生健康委科技计划项目(No.202211426);江西中医药大学2021年度上半年博士科研启动基金课题(No.2021BSZR011)。

摘　　要：目的探讨乌梅抗肝纤维化(HF)的作用及机制。方法将雄性SD大鼠随机分为正常对照组(n=10)与造模组(n=50),造模组大鼠以四氯化碳诱导建立HF模型。将成模大鼠分为模型组(生理盐水)、阳性对照药组[秋水仙碱,0.09 mg/(kg·d)]及乌梅低、中、高剂量组[1.35、2.70、5.40 g/(kg·d)],每组9只。大鼠每天灌胃相应药物/生理盐水1次,持续8周。末次给药后,计算大鼠肝脏指数,检测其肝功能指标、肝纤维化四项、氧化应激指标及炎症因子水平,采用苏木精-伊红(HE)染色法观察大鼠肝组织病理改变、Masson染色法观察大鼠HF程度、透射电镜法观察大鼠肝组织超微结构、TUNEL染色法检测大鼠肝细胞凋亡水平,并采用Western blot法检测大鼠肝组织中转化生长因子β1(TGF-β1)、血小板源性生长因子(PDGF)蛋白的表达。结果与正常对照组相比,模型组大鼠丙氨酸转氨酶、碱性磷酸酶、天冬氨酸转氨酶、总胆红素、丙二醛、Ⅲ型前胶原蛋白、Ⅳ型前胶原酶、层粘连蛋白、透明质酸、白细胞介素6、肿瘤坏死因子α水平和TGF-β1、PDGF蛋白表达水平均显著升高(P<0.01),超氧化物歧化酶、谷胱甘肽过氧化酶水平均显著降低(P<0.01);HE、Masson染色及透射电镜观察结果均提示模型组大鼠出现了明显的HF特征。与模型组比较,乌梅各剂量组大鼠上述指标均有不同程度改善,其中乌梅中、高剂量组大鼠上述指标均被显著逆转(P<0.05或P<0.01)。结论乌梅具有抗HF的作用,该作用可能是通过抗氧化、抗炎、减少胶原产生、抑制PDGF表达及调控TGF-β1信号通路等机制实现的。OBJECTIVE To explore the effect and mechanism of Prunus mume against hepatic fibrosis(HF).METHODS Male SD rats were randomly divided into normal control group(n=10)and modeling group(n=50).The modeling group established HF model using carbon tetrachloride.The modeled rats were randomly divided into model group(normal saline),positive control group[colchicine,0.09 mg/(kg·d)],and P.mume low-dose,medium-dose and high-dose groups[1.35,2.70,5.40 g/(kg·d)],with 9 rats in each group.They were given the corresponding drug/normal saline intragastrically,once a day,for 8 consecutive weeks.After the last medication,the liver index was calculated,while liver function indexes,liver fiber indexes,oxidative stress indicators and inflammatory factors of rats were measured.HE staining was used to observe the pathological changes in liver tissue of rats;Masson staining was used to observe the degree of HF in liver tissue of rats;transmission electron microscopy was used to observe the ultrastructure of liver tissue in rats;TUNEL staining was used to detect liver cell apoptosis in each group of rats.Western blot method was used to detect the protein expressions of transforming growth factor-β1(TGF-β1)and platelet-derived growth factor(PDGF)in liver tissue of rats.RESULTS Compared with normal control group,the levels of alanine transaminase,alkaline phosphatase,aspartate transaminase,total bilirubin,malondialdehyde,procollagen typeⅢprotein,Ⅳ-type pre collagenase,laminin,hyaluronic acid,interleukin-6,tumor necrosis factor-α,as well as the protein expressions of TGF-β1 and PDGF in model group were increased significantly,while the levels of superoxide dismutase and glutathione peroxidase were significantly reduced(P<0.01);the HE,Masson staining and transmission electron microscopy observation results showed obvious HF characteristics in rats of model group.Compared with model group,varying degrees of improvement in above indexes were observed in P.mume groups,and the above indicators of rats in P.mume medium-dose and high-do

关 键 词：乌梅 肝纤维化 转化生长因子β——1 血小板源性生长因子 

分 类 号：R285[医药卫生—中药学] R965[医药卫生—中医学]