多指标成分定量联合化学计量学、加权TOPSIS与灰色关联度融合模型评价不同产地飞扬草药材质量  

Fusion Model of Multi-index Component Quantitative Combined with Chemometrics,Weighted TOPSIS and Grey Relational Analysis for Quality Evaluation of Euphorbia hirta from Different Regions

作　　者：梁美锋 廖念 朱珊珊 王志坚 万雄飞 LIANG Meifeng;LIAO Nian;ZHU Shanshan;WANG Zhijian;WAN Xiongfei(Department of Pharmacy,China Resources&WISCO Genaral Hospital Affiliated to Wuhan University of Science and Technology,Wuhan 430080 Hubei,China;School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430070 Hubei,China)

机构地区：[1]武汉科技大学附属华润武钢总医院药学部,湖北武汉430080 [2]湖北中医药大学药学院,湖北武汉430070

出　　处：《中药新药与临床药理》2025年第1期125-133,共9页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：湖北省卫生健康委员会中医药科研项目(ZY2019Q014)。

摘　　要：目的建立多指标成分定量检测方法,联合化学计量学、加权逼近理想解排序(TOPSIS)与灰色关联度分析(GRA)融合模型对不同产地飞扬草药材质量进行综合评价。方法采用高效液相色谱(HPLC)法,以BetaMaxBaseC18柱为色谱柱,乙腈-0.2%磷酸为流动相梯度洗脱,检测波长分别为280nm(没食子酸、香草酸、丁香酸、松脂素)、360 nm(杨梅苷、异槲皮苷、槲皮苷、阿福豆苷)和210 nm(β-香树脂醇、β-谷甾醇、豆甾醇),同时检测16批飞扬草中没食子酸、香草酸、丁香酸、松脂素、杨梅苷、异槲皮苷、槲皮苷、阿福豆苷、β-香树脂醇、β-谷甾醇和豆甾醇含量,并按照《中国药典》方法检测16批飞扬草浸出物和总灰分含量。采用化学计量学、加权TOPSIS与GRA融合技术对检测数据进行分析,综合评价不同产地飞扬草药材质量差异。结果HPLC多指标成分定量检测方法操作便捷,专属性强,精密度、重复性及供试品溶液24 h内稳定性良好(RSD<2.0%);没食子酸、香草酸、丁香酸、松脂素、杨梅苷、异槲皮苷、槲皮苷、阿福豆苷、β-香树脂醇、β-谷甾醇和豆甾醇的线性关系良好(R2>0.999);相对校正因子耐用性良好(RSD<2.0%),一测多评(QAMS)法与外标(ESM)法结果接近(P>0.05);16批飞扬草中上述11个成分含量范围分别为0.315~0.540、0.382~0.731、0.127~0.245、0.055~0.114、2.605~4.368、0.164~0.390、0.956~1.603、0.090~0.155、0.024~0.071、0.038~0.104、0.041~0.079 mg·g^(-1);浸出物和总灰分范围分别为11.4%~19.1%、4.12%~9.33%。化学计量学结果显示16批飞扬草样品分3类,呈现一定产地差异;杨梅苷、香草酸、槲皮苷和异槲皮苷是影响飞扬草质量的主要差异性标志物。加权TOPSIS与灰色关联度融合模型相对贴近度0.3048~0.6394,产品质量优劣分类结果与化学计量学分类结果基本一致。结论基于多指标成分定量联合化学计量学、加权TOPSIS与GRA融合模型能有效地评�Objective To establish the quantitative detection method of multi-index component content of Euphorbia comprehensive evaluate the quality of Euphorbia hirta from different producing areas using fusion model chemometrics,weighted technique for order preference by similarity to an ideal solution(TOPSIS)and grey relational analysis(GRA).Methods The HPLC method was used with BetaMax Base C18 column.Acetonitrile-0.2%phosphoric acid was used as the mobile phase for gradient elution.The detection wavelengths were 280 nm(gallic acid,vanillic acid,syringic acid and pinoresinol),360 nm(myricitrin,isoquercitrin,quercitrin and afzelin),and 210 nm(β-amyrin,β-sitosterol and stigmasterol).At the same time,the contents of gallic acid,vanillic acid,syringic acid,pinoresinol,myricitrin,isoquercitrin,quercitrin,afzelin,β-amyrin,β-sitosterol and stigmasterol in 16 batches of Euphorbia hirta were detected.The contents of extract and total ash in Euphorbia hirta were detected according to Chinese pharmacopoeia.Fusion technology of chemometrics,weighted TOPSIS and GRA was used to analyze the test data,and the quality differences of Euphorbia hirta from different producing areas were comprehensively evaluated.Results The quantitative HPLC method of multi-index component was convenient and specific.The precision,repeatability and stability of the test solution within 24 h were good(RSD<2.0%).The linear relationship of gallic acid,vanillic acid,syringic acid,pinoresinol,myricitrin,isoquercitrin,quercitrin,afzelin,β-amyrin,β-sitosterol and stigmasterol were good(R2>0.999).The relative correction factor showed good durability(RSD<2.0%).The results of quantitative analysis of multi-components by single-marker(QAMS)method and external standard method(ESM)were close(P>0.05).The content ranges of the above 11 components in 16 batches of Euphorbia hirta were 0.315~0.540,0.382~0.731,0.127~0.245,0.055~0.114,2.605~4.368,0.164~0.390,0.956~1.603,0.090~0.155,0.024~0.071,0.038~0.104,0.041~0.079 mg·g^(-1).The content ranges of extract and total a

关 键 词：飞扬草 高效液相色谱法 一测多评法 化学计量学 逼近理想解排序 灰色关联度分析 质量评价 

分 类 号：R284.1[医药卫生—中药学]