血清tRNA-Gly-GCC-1-1在干燥综合征诊断中的临床价值  

Clinical value of serum tRNA-Gly-GCC-1-1 in the diagnosis and therapy of Sjögren′s syndrome

作　　者：史波 陈菲 刘文静 杨平 李智洋 SHI Bo;CHEN Fei;LIU Wenjing;YANG Ping;LI Zhiyang(Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University,Nanjing 210011,Jiangsu;Department of Clinical Laboratory,Nanjing Jiangning Hospital of Chinese Medicine,Nanjing 211100,Jiangsu,China)

机构地区：[1]南京医科大学鼓楼临床医学院&南京鼓楼医院检验科,南京210011 [2]南京市江宁中医院检验科,南京211100

出　　处：《临床检验杂志》2025年第1期25-29,共5页Chinese Journal of Clinical Laboratory Science

基　　金：鼓楼医院临床培育课题资助项目(2022-LCYJ-PY-36)。

摘　　要：目的检测并鉴定干燥综合征(SS)患者血清样本中差异表达的tRNA来源的小RNA(tsRNA)标志物,初步探究其在SS患者临床诊断中的潜在应用价值。方法收集南京鼓楼医院风湿科确诊为SS的患者以及年龄、性别相匹配的健康人对照血清样本进行小RNA测序,筛选出差异表达的tsRNA。使用实时荧光定量PCR(qRT-PCR)在2组研究对象血清样本中进行验证。采用ROC曲线评估其诊断SS的临床效能。使用KEGG及GO富集分析预测tRNA-Gly-GCC-1-1在SS发生、发展过程中的潜在机制。结果与健康人对照组[13.34(10.45,18.74)fmol/L]相比,SS组血清样本中tRNA-Gly-GCC-1-1[8.82(7.26,12.20)fmol/L]水平显著降低(P<0.0001),其区分SS患者及健康人对照的ROC曲线下面积(AUC^(ROC))为0.766(95%CI:0.671~0.861),当cut-off值为9.97 fmol/L时,其敏感性为83.33%,特异性为64.58%:生物信息分析提示血清tRNA-Gly-GCC-1-1参与了PI3K-Akt等信号通路的调控。结论tRNA-Gly-GCC-1-1在SS患者血清样本中呈显著低表达,具有潜在辅助诊断SS的临床应用价值。Objective To detect and identify the differentially expressed tsRNA in the serum of patients with Sjögren′s syndrome(SS),and preliminarily explore their potential application value in the diagnosis of SS.Methods The serum samples from SS patients diagnosed at the Rheumatology Department of Nanjing Drum Tower Hospital and healthy controls(HCs)matched age and gender were collected,and the differentially expressed tsRNA were screened by small RNA sequencing.Fluorescence quantitative real-time PCR(qRT-PCR)was used to valid the sequence data.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficiency of tRNA-Gly-GCC-1-1.KEGG and GO analysis were used to predict the potential mechanism of tRNA-GLy-GCC-1-1 in the occurrence and development of SS.Results Compared with HCs(13.34[10.45,18.74]fmol/L),the levels of serum tRNA-Gly-GCC-1-1 in SS patients(8.82[7.26,12.20]fmol/L)were significantly reduced(P<0.0001),and its area under the ROC curve(AUC^(ROC))in distinguishing SS patients from HCs was 0.766(95%CI:0.671-0.861).When the cut-off value was 9.97 fmol/L,its sensitivity and specificity were 83.33%and 64.58%,respectively.Bioinformatics analysis showed that serum tRNA-Gly-GCC-1-1 might be involved in the regulation of signaling pathways such as PI3K-Akt.Conclusion Serum tRNA-Gly-GCC-1-1 in SS patients is significantly down-regulated,which has potential clinical application value in assisting the diagnosis of SS.

关 键 词：干燥综合征 RNA测序 tRNA来源的小RNA 生物学标志物 

分 类 号：R446[医药卫生—诊断学]