基因编辑技术的研究进展  

Research Progress in Gene Editing Technology

作　　者：包斌武 邹惠影 李俊良 高晨 高会江[2] 杜振伟 张博玉 李俊雅 高雪[2] BAO Binwu;ZOU Huiying;LI Junliang;GAO Chen;GAO Huijiang;DU Zhenwei;ZHANG Boyu;LI Junya;GAO Xue(College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]宁夏大学动物科技学院,银川750021 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2025年第1期1-14,共14页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：科技创新2030—重大项目(2023ZD04048,2023ZD0404801)。

摘　　要：基因编辑技术是修饰特定基因的新型分子工具,可在基因序列中有效引入新的突变,为分析基因功能和与表型相关的DNA序列提供了有效的手段。自发现以来,基因编辑技术已经成功应用在各种动物、植物及其他生物中,为基因治疗及精准作物育种等领域提供了重要技术支撑。本文旨在回顾近年来基因编辑工具的发展,阐述了经典编辑工具、碱基编辑工具和其他新编辑工具的进展,以期为相关领域科研人员进一步了解、优化编辑系统提供参考。Gene editing technology is a novel molecular tool for modifying specific genes,which can effectively introduce new mutations into gene sequences,providing effective means for analyzing gene function and the phenotype-related DNA sequences.Since its discovery,gene editing technology has been successfully applied in various animals,plants,and other organisms,providing important technical support for gene therapy and precision crop breeding.This article aims to review the development of gene editing tools in recent years,elucidate the progress of classic editing tools,base editing tools,and other new editing tools,in order to provide reference for researchers in related fields to further understand and optimize editing systems.

关 键 词：基因编辑 ZFN编辑 TALENs编辑 CRISPR-Cas 碱基编辑 

分 类 号：S813.1[农业科学—畜牧学]