SERS@Au微阵列芯片快速检测细菌性结膜炎病原体  

Rapid Detection of Bacterial Conjunctivitis Pathogens Using SERS@Au Microarray Chip

作　　者：刘文博[1] 李含 徐瑗聪 刘梦东 王惠琴[1] 林太凤[1] 郑大威[1] 张萍[1] LIU Wen-bo;LI Han;XU Yuan-cong;LIU Meng-dong;WANG Hui-qin;LIN Tai-feng;ZHENG Da-wei;ZHANG Ping(College of Chemistry and Life Sciences,Beijing University of Technology,Beijing 100124,China)

机构地区：[1]北京工业大学化学与生命科学学院,北京100124

出　　处：《光谱学与光谱分析》2025年第2期476-482,共7页Spectroscopy and Spectral Analysis

基　　金：国家重点研发计划项目(2017YFC0111104);北京市教委科研计划项目(KM201810005031)资助。

摘　　要：急性细菌性结膜炎是一种常见的眼科疾病,医治不及时会严重损害视力。目前针对细菌性结膜炎常规的诊断方法仍为微生物培养法,该方法灵敏度高,但耗时耗力,难以满足快速检测的需求。该研究开发了一种SERS@Au微阵列芯片,将其作为增强基底收集细菌性结膜炎相关金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和表皮葡萄球菌的SERS图谱。结果表明,该芯片具有良好的增强效果、重现性和稳定性。选取400~1800 cm^(-1)波段,通过建立SVM模型和OPLS-DA模型对四种致病菌进行判别分析,区分准确率分别达到97%和90%。采用SERS@Au微阵列芯片对加标泪液进行检测,仅需短暂培养即可快速、准确、便捷、无损筛查致病菌,减少患者的痛苦。该研究开发的SERS@Au微阵列芯片与便携式拉曼光谱仪配套使用,具有准确、便携、快速、现场检测及微量样品检测的特点,适用于眼科细菌性感染疾病的快速筛查。该方法无需标记、无需鉴定培养基、对患者无损,实现了对复杂生物样本混合感染的快速检测,大大提高了检测效率,有望成为眼科疾病的新型辅助筛查手段。Acute bacterial conjunctivitis is a prevalent ocular disease that can lead to severe vision impairment if not promptly treated.The conventional diagnostic method for bacterial conjunctivitis still relies on microbial culture,which,although highly sensitive,is time-consuming,labor-intensive,and unable to meet the demand for rapid detection.In this study,we developed a SERS@Au microarray chip as an enhanced substrate for collecting the SERS spectra of conjunctivitis-associated bacteria,including Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa,and Staphylococcus epidermidis.The results demonstrated that the SERS@Au microarray chip has an excellent enhancement effect,reproducibility,and stability.By selecting the Raman shifts from 400 to 1800 cm^(-1) of pathogenic bacteria to perform an SVM model and OPLS-DA model,the discrimination accuracies achieved 97% and 90%,respectively.Detecting spiked tears using the SERS@Au microarrays chip can quickly,accurately,and conveniently screen pathogenic bacteria with simple culture.Collecting tears for direct testing is simple,painless,and non-destructive for patients.Combining the SERS@Au microarray with a portable Raman spectrometer is suitable for on-site screening of ophthalmic bacterial infections.Moreover,it enables the detection of mixed infections,thereby greatly enhancing overall efficiency in diagnosis and providing a valuable assistive tool for ophthalmic disease screening.

关 键 词：表面增强拉曼光谱 微阵列芯片 细菌性结膜炎 正交偏最小二乘判别分析 支持向量机 快速检测 

分 类 号：Q939.93[生物学—微生物学] R319[医药卫生—基础医学]