复方排石汤剂对草酸钙肾结石模型大鼠保护作用实验研究  

Experimental Study on Compound Paishi Decoction for Calcium Oxalate Kidney Stone Model Rats

作　　者：狄俊俊 方宗潮 黄敏聪 DI Junjun;FANG Zongchao;HUANG Mincong(Taizhou Hospital of Integrated Chinese and Western Medicine,Taizhou Zhejiang 317200,China;Hangzhou Medical College,Hangzhou Zhejiang 310000,China)

机构地区：[1]台州市中西医结合医院,浙江台州317200 [2]杭州医学院,浙江杭州310000

出　　处：《新中医》2025年第2期176-181,共6页New Chinese Medicine

基　　金：温岭市科技项目(2019S0180023)。

摘　　要：目的:观察复方排石汤对草酸钙肾结石模型大鼠的药效,并初步探索其可能机制。方法:将42只雄性SD大鼠随机分为6组,即正常对照组、模型对照组、阳性对照组、复方排石汤剂低(0.26g·mL-1)、中(0.52g·mL-1)、高(1.03g·mL-1)剂量组。采用乙二醇和氯化铵试剂联合诱导SD大鼠草酸钙肾结石实验模型,造模同时正常对照组及模型对照组给予饮用水灌胃,阳性对照组给予25%枸橼酸钾溶液灌胃,复方排石汤高、中、低剂量组分别给予复方排石汤剂低、中、高剂量灌胃,干预28 d。试验期间通过观测大鼠体质量等一般体征,检测大鼠血清尿素氮(BUN)、肌酐(SCr)、血钙(Ca2+)以及尿液中尿素氮、肌酐含量及肾组织病理变化程度;评价肾组织中p38 MAPK信号通路的激活[p38磷酸化程度(p-p38/p38)、转化生长因子-β1(TGF-β1)、P47-phox水平]情况。结果:4周后,模型对照组大鼠血清SCr和BUN水平较正常对照组升高(P<0.05)。与模型对照组比较,阳性对照组大鼠给予枸橼酸钾治疗后血清SCr、BUN水平降低(P<0.05);复方排石汤剂中剂量组和复方排石汤剂高剂量组大鼠血清SCr、BUN水平降低(P<0.05),复方排石汤剂低剂量组大鼠血清SCr、BUN水平有所降低,但差异无统计学意义(P>0.05)。4周后,模型对照组大鼠尿Ca2+和尿草酸含量较正常对照组升高(P<0.05),血Ca2+浓度未见显著改变(P>0.05)。与模型对照组比较,阳性对照组大鼠尿Ca2+和尿草酸含量降低(P<0.05);复方排石汤剂中剂量组和复方排石汤剂高剂量组大鼠尿Ca2+和尿草酸含量降低(P<0.05),复方排石汤剂低剂量组大鼠尿草酸含量降低(P<0.05)。4周后,模型对照组大鼠外周血NEUT比例较正常对照组升高(P<0.05),LYMPH比例较正常对照组降低(P<0.05)。与模型对照组比较,阳性对照组大鼠NEUT比例降低(P<0.05),LYMPH比例升高(P<0.05);复方排石汤剂中剂量组和复方排石汤剂高剂量组大鼠NEUT比例降低(P<0.05)Objective:To observe the therapeutic effect of Compound Paishi Decoction on calcium oxalate kidney stone model rats,and to explore its possible mechanism.Methods:A total of 42 male SD rats were randomly divided into six groups:the normal control group,the model control group,the positive control group,the low-,medium-and high-dose groups(0.26 g·mL-1,0.52 g·mL-1,1.03 g·mL-1)of Compound Paishi Decoction.The experimental model SD rats with calcium oxalate kidney stone were induced by ethylene glycol and ammonium chloride reagent,and the models were simultaneously treated with low-,medium-and high-doses of Compound Paishi Decoction by continuous instillation for 28 days.During the experiment,the body weight and other general signs of rats were observed,and the contents of blood urea nitrogen(BUN),creatinine(SCr)and blood calcium(Ca2+)in serum,and urea nitrogen and creatinine in urine as well as the pathological changes of renal tissue were detected.The activation of p38 MAPK signaling pathway[p38 phosphorylation degree(p-p38/p38),transforming growth factor-β1(TGF-β1),P47-phox levels]in renal tissue were evaluated.Results:After four weeks,serum SCr and BUN levels in the model control group were increased when compared with those in the normal control group(P<0.05).Compared with those in the model control group,serum SCr and BUN levels in the positive control group were significantly decreased after potassium citrate treatment(P<0.05);serum SCr and BUN levels in the medium-dose group and the high-dose group of Compound Paishi Decoction were significantly decreased(P<0.05),and serum SCr and BUN levels in the low-dose Compound Paishi Decoction group were decreased,but there was no significant difference(P>0.05).After four weeks,the contents of urinary Ca2+and urinary oxalate in the model control group were increased when compared with those in the normal control group(P<0.05),but the concentration of blood Ca2+was not significantly changed(P>0.05).Compared with those in the model control group,the contents of urine

关 键 词：肾结石 草酸钙 复方排石汤 肾功能 p38 MAPK信号通路 

分 类 号：R285.5[医药卫生—中药学] R692.4[医药卫生—中医学]