芦根多糖的提取、结构表征及对巨噬细胞免疫抑制作用研究  

Extraction,structural characterization and immunosuppression on macrophages of polysaccharides from Phragmites rhizome

作　　者：崔明晓 方舟 宫霞[1] 黄玥[1] 周睿美 刘克海[2] 白晨[1] CUI Mingxiao;FANG Zhou;GONG Xia;HUANG Yue;ZHOU Ruimei;LIU Kehai;BAI Chen(Department of Food Science,Shanghai Business School,Shanghai 200235,China;College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;School of Medicine,Tongji University,Shanghai 200092,China)

机构地区：[1]上海商学院食品系,上海200235 [2]上海海洋大学食品学院,上海201306 [3]同济大学医学院,上海200092

出　　处：《上海海洋大学学报》2024年第6期1439-1450,共12页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(82172796);2022年度上海高校市级重点课程“食品营养学”建设项目(2436611006)。

摘　　要：基于微波辅助提取法提取芦根多糖,表征其结构、评价其体外免疫抑制功效。采用响应面法优化芦根多糖的提取条件,获得最佳分离条件为微波功率572 W、微波时间22 min、液料比15 mL/g,芦根多糖得率为1.61%。芦根多糖的单糖组成为阿拉伯糖、半乳糖、葡萄糖,摩尔比为3.03∶0.52∶6.46。红外分析发现芦根多糖中可能含有糖醛酸及硫酸基团。芦根多糖溶液为无色透明液体。扫描电镜发现芦根多糖表面光滑且结构致密,呈不规则且厚度不均匀的片状结构。细胞试验分析发现25~300μg/mL芦根多糖能够显著提高巨噬细胞的细胞活性,该多糖组与空白组无显著性差异。5~200μg/mL芦根多糖能够显著抑制脂多糖刺激导致的巨噬细胞中一氧化氮的产生。100μg/mL芦根多糖能显著抑制脂多糖刺激导致的巨噬细胞中肿瘤坏死因子α及白细胞介素6的产生。因此,芦根多糖可能具有良好的免疫抑制功效。In this study,microwave-assisted extraction was used for the isolation of Phragmites rhizoma polysaccharide,its structure was characterized and its immunosuppressive effects in vitro were evaluated.The response surface method was used to optimize the extraction of polysaccharide.The optimal separation conditions of Phragmites rhizoma polysaccharide were microwave power 572 W,microwave time 22 min,liquid-solid ratio 15 mL/g,and the yield of Phragmites rhizoma polysaccharide was 1.61%.The monosaccharide composition of Phragmites rhizoma polysaccharide was arabinose,galactose and glucose,with a molar ratio of 3.03∶0.52∶6.46.Infrared analysis showed that Phragmites rhizoma polysaccharide may contain uronic acid and sulfuric acid groups.Phragmites rhizoma polysaccharide solution is colorless and transparent liquid.Scanning electron microscopy showed that Phragmites rhizoma polysaccharide was smooth and compact,with irregular sheet structure and uneven thickness.Cell analysis showed that 25−300μg/mL Phragmites rhizoma polysaccharide could significantly improve the cell activity of macrophages and there was no significant difference between the polysaccharide groups and the blank group.5−200μg/mL Phragmites rhizoma polysaccharide significantly inhibited nitric oxide production in lipopolysaccharide-stimulated macrophages.100μg/mL Phragmites rhizoma polysaccharide significantly inhibited the production of inflammatory cytokines tumor necrosis factorαand interleukin 6 in macrophages stimulated by lipopolysaccharide.Therefore,Phragmites rhizoma polysaccharide may have good immunosuppressive effects.

关 键 词：芦根多糖 多糖得率 响应优化 结构表征 免疫抑制 

分 类 号：R284[医药卫生—中药学]