ARF4 acting upstream of LBD16 promotes adventitious root formation in peach  

作　　者：Jingjing Liu Lingling Gao Ruoxi Zhang Anqi Gao Zamith Kerubo Oginga Beibei Zheng Yuepeng Han 

机构地区：[1]CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,The Innovative Academy of Seed Design of Chinese Academy of Sciences,Wuhan,Hubei 430074,China [2]Hubei Hongshan Laboratory,Wuhan,Hubei 430070,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Horticultural Plant Journal》2025年第1期145-161,共17页园艺学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.32272690 and 32272687);the China Agriculture Research System(Grant No.CARS-30);Hubei Hongshan Laboratory(Grant No.2021hszd017)。

摘　　要：Although class A auxin response factors(ARFs)are known to regulate adventitious root(AR)development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway,the regulatory role of class B ARFs in AR development remains largely unclear.Therefore,this research focused on the role of class B ARF transcription factors in peach(Prunus persica‘Shengli')adventitious root formation.Here,we report the role of a class B ARF gene Pp ARF4 in adventitious root formation in peach.Comparative transcriptome and q RT-PCR analyses showed that the transcription of Pp ARF4 was significantly up-regulated in auxin-treated stem explants.Y2H assay showed that Pp ARF4 had no interaction with Pp IAAs(AUXIN/INDOLE ACETIC ACIDs).Pp ARF4 could bind the promoters of lateral root development gene Pp LBD16 and auxin transport gene Pp PIN1 to activate their transcription.Ectopic overexpression of Pp ARF4 and Pp LBD16 in Arabidopsis promoted AR development.Additionally,Pp ARF4 could act as a negative regulator of flavone synthesis and thus prevent the explants from browning.The results not only provide novel insights into the functions of ARFs in regulating plant growth and development,but will also be useful for fulfilling asexual propagation by stem cuttings in peach.

关 键 词：Prunus persica Adventitious root AUXIN Pp ARF4 Pp LBD16 

分 类 号：S662.1[农业科学—果树学]