Compiling molecular evidence from a tetraploid rose genome into a near-saturated map for the identification of pigment-related genes  

作　　者：Bixuan Cheng Yanlin Sun Huihua Wan Kai Zhao Lidan Sun Le Luo Huitang Pan Qixiang Zhang Chao Yu 

机构地区：[1]State Key Laboratory of Efficient Production of Forest Resources,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment and School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China [2]Key Laboratoryof Beijing for Identification and Safety Evaluation of Chinese Medicine,Instituteof Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China

出　　处：《Horticultural Plant Journal》2025年第1期351-366,共16页园艺学报(英文版)

基　　金：supported by National Natural Science Foundation of China(Grant No.32071818)。

摘　　要：With their high economic value and cultural significance,modern roses are one of the most important ornamental plants.Because of their complicated genetic background and tetraploid nature,the creation of high-density genetic maps of roses has been a challenge that has slowed the pace of molecular breeding for modern roses.The current construction of tetraploid genetic maps based on existing diploid rose genomes could lead to inaccurate marker information and genotyping results.Therefore,we generated the first high-quality tetraploid genome of Rosa chinensis‘Yunzheng Xiawei.'Utilizing Illumina,PacBio,and Hi-C sequencing technologies,we assembled a genome of 858.59 Mb with 14pseudo-chromosomes.Mode of inheritance analysis using PolyOrigin indicated that modern roses show both quadrivalent and bivalent pairing.Based on this reference genome,high-density genetic maps were constructed using MSTmap with nearly saturated markers.Quantitative trait locus(QTL)analysis was conducted using WinQTLCart and R/qtl for flavonoids and carotenoids,and 11 QTL clusters were identified.By combining the genome annotation,phylogenetic analyses,and gene expression analyses,we were able to identify several key genes related to flavonoid and carotenoid biosynthesis.This study provides the basis for further genetic analyses of highly heterozygous tetraploid roses and could facilitate the progress of marker-assisted selection in modern roses.

关 键 词：ROSA Tetraploid genome Tetrasomic inheritance Genetic linkage map Flower color 

分 类 号：S685.12[农业科学—观赏园艺]