NaCl inhibits Geotrichum citri-aurantii growth by inducing lipid droplets accumulation and enhances the biocontrol efficacy of Kloeckera apiculata 34-9  

作　　者：Juan Zhao Yuqing Wang Qianyi Liu Chaoan Long 

机构地区：[1]Faculty of Food Science and Engineering,Kunming University of Science and Technology,Kunming,Yunnan 650500,China [2]National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,National R&D Center for Citrus Preservation,National Centre of Citrus Breeding,Huazhong Agricultural University,Wuhan,Hubei 430070,China [3]Shenzhen Institute of Nutrition and Health,Huazhong Agricultural University,Shenzhen,Guangdong 518000,China [4]Shenzhen Branch,Guangdong Laboratoryfor Lingnan Modern Agriculture,Genome Analysis Laboratoryof the Ministryof Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518000,China

出　　处：《Horticultural Plant Journal》2025年第1期455-458,共4页园艺学报(英文版)

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.32302170&32172255);HZAU-AGIS Cooperation Fund(Grant No.SZYJY2023001)。

摘　　要：Decays resulting from infections by Geotrichum citri-aurantii cause a significant reduction in citrus postharvest quality and marketable yield.In the present work,0.8 mol·L^(-1)NaCl of Generally Recognized as Safe(GRAS)salts were identified to have complete inhibition activity to G.citriaurantii in vitro and in vivo.An inhibitory mechanism study showed that 0.1 mol·L^(-1)NaCl changed the expression of six genes(Gci002087,Gci003354,Gci000013,Gci000394,Gci003505 and Gci004433)in target of rapamycin(TOR)pathway,thus inducing a large number of lipid droplets(LDs)accumulation with TEM observations and staining.In vivo,0.1 mol·L^(-1)NaCl could raise up the activities of CAT,PAL,PPO and cellulase which hence to strengthen the ability of resistance tolerance of citrus.In addition,NaCl not only accelerated the growth of the biocontrol yeast Kloeckera apiculata strain 34-9,but also increased the expression level of adhesin-encoding genes and biofilm-formation encoding gene(34-9-3691).Finally,the storage experiments in 3 months demonstrated that the combination of NaCl and the yeast 34-9 could keep fresh,reduce decay and prolong its shelf-life,thus implying that the combination of biocontrol strain and GRAS salts has been explored as a promising alternative to synthetic fungicides.

关 键 词：NACL Geotrichum citri-aurantii Kloeckera apiculata LDs Postharvest preservation CITRUS 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]