纳米石墨适配体传感器结合酶信号放大检测甲胎蛋白  

Nano-graphite aptasensor combined with enzyme signal amplification for the detection of alpha-fetoprotein

作　　者：张凤娇 贾超凡 徐新秋 闫业浩 公维磊[2] 张凯[2] 魏胤 ZHANG Feng-jiao;JIA Chao-fan;XU Xin-qiu;YAN Ye-hao;GONG Wei-lei;ZHANG Kai;WEI Yin(School of Public Health,Shandong First Medical University and Shandong Academy of Medical Sciences,Jinan 250117,China;Key Laboratory of Food Nutrition and Safety,School of Public Health,Jining Medical College,Jining 272000,China;School of Public Health,Binzhou Medical College,Yantai 264000,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,山东济南250117 [2]济宁医学院公共卫生学院食品营养与安全重点实验室,山东济宁272000 [3]滨州医学院公共卫生学院,山东烟台264000

出　　处：《化学研究与应用》2025年第1期81-86,共6页Chemical Research and Application

基　　金：山东省自然科学基金青年项目(ZR2020QH297)资助。

摘　　要：利用纳米石墨-适配体传感器结合脱氧核糖核酸酶(DNase I)信号放大策略检测了肿瘤标志物甲胎蛋白(AFP)。通过将纳米石墨(NG)和DNase I加入到适体(aptamer)溶液中合成了一种新型的荧光传感器NG/Aptamer。加入AFP后,AFP/aptamer复合物的形成导致aptamer从NG表面脱离,随后aptamer被DNase I分解,目标AFP被释放出来进行新一轮循环,从而实现了检测信号的明显放大。在最佳条件下,AFP浓度在3~150 pg·mL^(-1)范围内与荧光强度呈线性关系,检测限为2.556 pg·mL^(-1)。此外,所开发的传感器对AFP具有高度特异性,不会受到其他结构类似物质的干扰。该传感器也可应用于人血清样本中AFP的检测,回收率为95.1~103.8%。The tumor marker alpha-fetoprotein(AFP)was detected using a graphite-aptamer sensor combined with DNase I signal amplification strategy.A novel fluorescence sensor,NG/Aptamer,was synthesized by incorporating nano-graphite(NG)and DNase I into the aptamer solution.Following the addition of AFP,the formation of the AFP/aptamer complex led to the dissociation of the aptamer from the NG surface.The aptamer was then degraded by DNase I,releasing the target AFP for a new cycle,resulting in a significant amplification of the detection signal.Under optimal conditions,the AFP concentration within the range of 3~150 pg·mL^(-1)exhibited a linear relationship with fluorescence intensity.The detection limit was established at 2.556 pg·mL^(-1).Furthermore,the developed sensors demonstrated high specificity for AFP,with no interference from other structurally similar substances.The sensor was also applicable for AFP detection in human serum samples,achieving a recovery rate between 95.1%and 103.8%.

关 键 词：纳米石墨 甲胎蛋白 适配体 

分 类 号：O657.3[理学—分析化学]