两种AMF组合对石蒜叶生长及光合效能的影响  

Effects of two AMF combinations on leaf growth and photosynthetic efficiency of Lycoris radiata

作　　者：温婷 游欣 吴靖 魏绪英[2] 王琼 蔡军火[1] WEN Ting;YOU Xin;WU Jing;WEI Xuying;WANG Qiong;CAI Junhuo(College of Landscape Architecture,Jiangxi Agricultural University,Nanchang 330045,China;College of Art,Jiangxi Finance and Economics University,Nanchang 330032,China)

机构地区：[1]江西农业大学园林与艺术学院,江西南昌330045 [2]江西财经大学艺术学院,江西南昌330032

出　　处：《江西农业大学学报》2024年第6期1436-1444,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(32360420、31960327)。

摘　　要：【目的】旨在探究接种丛枝菌根真菌(AMF)对红花石蒜生长及光合效能的影响,筛选适宜其栽培的伴生菌种。【方法】将盆栽红花石蒜放在人工气候箱内进行培养(25/20℃),以摩西斗管囊霉(Funneliformis mosseae,FM)和根内根孢囊霉(Rhizophagus intraradices,RI)这2种AMF为试验菌剂,采用3种AMF接种处理(FM、RI、FM+RI),以不接种处理为对照(CK),分别统计其根系侵染率、叶片生长情况,并测定光合生理指标。【结果】(1)AMF能与红花石蒜形成良好的共生关系;3种AMF处理的平均根系侵染率为83.55%,以混合双接(FM+RI)处理最高(达87.00%)。(2)不同AMF接种处理对红花石蒜的叶片生长有显著的促进作用,其中FM、RI和FM+RI组的叶片长度最大值分别较CK增加了5.04%、6.85%和18.89%。(3)在25/20℃条件下,4种处理的叶片均始终处于“不断更新”状态。(4)3种AMF接种处理均能有效提高红花石蒜叶片的叶绿素相对含量、光能利用率以及气孔交换能力,并减少能量散失。其中,以FM+RI组效果最佳,其叶绿素相对含量、PSΠ的实际光合效率、光化学淬灭系数、光合电子传递速率、净光合速率、气孔导度和水分利用率分别较CK增加了9.89%、42.5%、25.91%、37.93%、50.00%、22.33%和75.53%,并显著降低了非光化学淬灭系数(54.68%)和胞间CO_(2)浓度(30.03%)。(5)不同接种处理对红花石蒜叶片生长及其光合效能的促进效应由好到差依次为FM+RI、FM、RI和CK。【结论】3种AMF处理均能有效侵染红花石蒜的根系,也能明显促进其叶片的伸长生长和光合效率的提高,其中以混接处理(FM+RI)的综合效果最好,可考虑在红花石蒜的产业化栽培中推广。[Objective]This study aims to examine the impact of inoculating Lycoris radiata with arbuscular mycorrhizal fungi(AMF)on its growth and photosynthetic efficiency,in order to screen good strains suitable for the cultivation of L.radiata.[Method]The Potted L.radiata was cultivated in an artificial climate chamber(25/20℃).Two types of AMF,Funneliformis mosseae(FM)and Rhizophagus intraradices(RI),were used as test inoculants,and three AMF inoculation treatments(FM,RI,and FM+RI)were adopted,with no inoculation treatment as the control(CK).The root infection rate,leaf growth and photosynthetic physiological indexes were measured respectively.[Result]The results showed that:(1)AMF can form a beneficial symbiotic relationship with Lycoris radiata.The average root infestation rate of the three AMF treatments was 83.55%,with the mixed double inoculation treatment(FM+RI)having the highest root infestation rate(up to 87%).(2)Different AMF inoculation treatments had a significant promoting effect on the leaf growth of L.radiata.Compared with the control group(CK),the average maximum leaf length in the FM,RI,and FM+RI groups increased by 5.04%,6.85%,and 18.89%,respectively.(3)Under the conditions of 25/20℃,the leaves of the four treatments were always in a“constantly renewing”state.(4)All three AMF inoculation treatments were effective in increasing the relative chlorophyll content,light energy utilisation and leaf stomatal exchange capacity of L.radiata,and reducing energy loss.Among them,FM+RI was the most effective,which increased the relative chlorophyll content,the actual photosynthetic efficiency of PSΠ,the photochemical quenching coefficient,the photosynthetic electron transfer rate,the net photosynthetic rate,the stomatal conductance,and the water utilisation by 9.89%,42.5%,25.91%,37.93%,50.00%,22.33%,and 75.53%,respectively,compared with that of CK.It also significantly reduced the non-photochemical quenching coefficient(54.68%)and intercellular CO_(2) concentration(30.03%).(5)The effects of different inocula

关 键 词：红花石蒜 AMF真菌 生长效应 光合特性 

分 类 号：S682.29[农业科学—观赏园艺]