寒区绿豆根瘤微生物组及优势慢生根瘤菌的分离、结瘤活性  

Nodules Microbiome of Mung Bean in Cold Regions and Isolation,Re-Inoculate Activity of Dominant Bradyrhizobium japonicumfrom Mung Bean Nodules

作　　者：张杰 陈永旗 施熠涵 刘文瑞 刘思宇 韩毅强[1] 高亚梅[1,2] ZHANG Jie;CHEN Yongqi;SHI Yihan;LIU Wenrui;LIU Siyu;HAN Yiqiang;GAO Yamei(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Key Laboratory of Environmental Microbiology and Agricultural Waste Resource Utilization in Cold Regions of Heilongjiang Province,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]黑龙江省寒区环境微生物与农业废弃物资源化利用重点实验室,黑龙江大庆163319

出　　处：《黑龙江农业科学》2025年第2期7-14,共8页Heilongjiang Agricultural Sciences

基　　金：2021年黑龙江省大学生创新创业项目(202110223053);黑龙江省面上基金项目(LH2021C065)。

摘　　要：为探究寒区绿豆根瘤的微生物组并获得绿豆的本地根瘤菌资源,利用高通量测序方法分析了黑龙江省大庆市安达试验田绿豆根瘤的微生物组,并利用平板划线法从绿豆根瘤中分离、鉴定优势根瘤菌,并开展了回接结瘤试验,考察了结瘤植株的生长情况。结果显示,绿豆根瘤中共检测到113个OTU,主要微生物菌群来自变形菌门、蓝藻门、放线菌门和未分类细菌门,优势的慢生根瘤菌属占64.29%,其他为非根瘤菌属。网络分析显示慢生根瘤菌属与6种非根瘤菌存在相关性,并与假单胞菌属存在正相关性。分离获得的优势根瘤菌利用16S rDNA进行分子鉴定结果表明,该根瘤菌与慢生根瘤菌(Bradyrhizobium japonicum)的相似性为99.93%。回接结瘤后的绿豆植株根长减少5.8%,但其株高以及鲜重增加39.7%和236.1%,地下鲜重提高111.4%。In order to investigate the nodules microbial community of mung bean in cold regions and obtain local rhizobium resources for mung beans,nodules microbiome of mung bean was analyzed using high-throughput sequencing methods,and a rhizobia was isolated and identified from nodules of mung bean in Anda experimental field,Daqing City,Heilongjiang Province by the plate streaking method.Moreover,we conducted a reinoculate test to investigate the growth of plants.The results showed that 113 OTUs were detected in the nodules of mung bean and the dominant phyla included Proteobacteria,Cyanobacteria,Actinobacteria,and Unclassified Bacteria;The dominant Bradyrhizobium genus accounted for 64.29%,while the others are non-rhizobia genera.The network analysis showed a correlation between Bradyrhizobium and six non-rhizobia,and a positive correlation between Bradyrhizobium and Pseudomonas.The molecular identification based on 16S rDNA showed the rhizobia had a 99.93%similarity with Bradyrhizobium japonicum.After nodulation,the root length of mung bean decreased by 5.8%,but the plant height and fresh weight increased by 39.7%and 236.1%,respectively.The underground fresh weight increased by 111.4%.This study analyzed the endophytic community structure of mung bean nodules and obtained a dominant Bradyrhizobium japonicum with growth promoting ability from the nodules.

关 键 词：绿豆 根瘤 微生物组 慢生根瘤菌 回接结瘤试验 

分 类 号：S522[农业科学—作物学]