手性金纳米探针用于药物分子对映体的比色检测  

Chiral Gold Nanoprobes for Colorimetric Detection of the Drug Enantiomers

作　　者：谭晓萍 吴宏伟 王丹丹 张金[1] 章俊辉 TAN Xiaoping;WU Hongwei;WANG Dandan;ZHANG Jin;ZHANG Junhui(Yunnan Key Laboratory of Modern Separation Analysis and Substance Transformation,College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南省现代分离分析与物质转化重点实验室,云南师范大学化学化工学院,昆明650500

出　　处：《高等学校化学学报》2025年第2期70-77,共8页Chemical Journal of Chinese Universities

基　　金：云南省基础研究计划项目(批准号:202401AT070128);云南省教育厅科学研究基金(批准号:2023j0204)资助.

摘　　要：基于金纳米粒子良好的光电特性,以及移动智能设备良好的RGB传感性能,设计了一种可用于快速检测R/S-苯丙氨醇、R/S-布洛芬对映体分子的手性比色检测方法.手性金纳米粒子识别对映体分子时表现出非共价相互作用,如静电、氢键和亲(疏)水相互作用等,使得纳米材料表面发生能量共振转移,导致其趋于稳定和聚集,进而显示出不同的颜色,最终实现比色检测的目的.在检测过程中加入铜离子(Cu^(2+)),由于Cu^(2+)能同时与对映体分子和手性金纳米粒子形成配合物,因此能够增强手性识别效果.此外,利用移动智能设备灵敏的RGB检测性能,实现了R/S-苯丙氨醇、R/S-布洛芬对映体分子的定量检测,其检测限分别为124.35和104.58μmol/L;123.95和111.44μmol/L.同时,该方法在一定的浓度范围内可实现对映体混合物的检测.本研究应用手性金纳米粒子作为比色探针,以移动智能设备为检测装置,构建了一种快速便捷的检测方法,在药物对映体分子检测方面具有潜在的应用价值.Based on the good optoelectronic properties of gold nanoparticles and the good RGB sensing performance of mobile intelligent devices,a chiral colorimetric detection method for the rapid detection of R/S-phenylalamine and R/S-ibuprofen enantiomer molecules was designed.Chiral gold nanoparticles exhibit non-covalent interactions,such as electrostatic,hydrogen bonding,and hydrophilic(hydrophobic)interactions,which cause energy resonance transfer on the surface of nanomaterials,resulting in their stabilization and aggregation,and then show different colors,and finally achieving the purpose of colorimetric detection.The addition of copper ions(Cu^(2+))during the detection process can enhance chiral recognition because Cu^(2+) can form complexes with enantiomer molecules and chiral gold nanoparticles at the same time.In addition,the sensitive RGB detection performance of mobile intelligent devices was used to quantitatively detect the enantiomer molecules of R/S-phenylalamine and R/S-ibuprofen,and the detection limits were 124.35 and 104.58μmol/L,123.95 and 111.44μmol/L,respectively.At the same time,the method can realize the detection of enantiomeric mixtures within a certain concentration range.In this work,chiral gold nanoparticles were used as colorimetric probes and mobile intelligent devices were used as detection devices to construct a fast and convenient detection method,which has potential application value in the detection of enantio⁃mer molecules.

关 键 词：药物分子对映体 金纳米粒子 手性识别 比色检测 RGB检测 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]