桑树桑黄多糖提取工艺优化及其细胞毒性检测  

Optimization of the Extraction of Polysaccharides and the Cytotoxicity Evaluation from Sanghuangporus sanghuang

作　　者：曲芸鹤 王锐 张平平 时东方[1] QU Yunhe;WANG Rui;ZHANG Pingping;SHI Dongfang(Central Laboratory,Changchun Normal University,Changchun 130032,China;Tonghua Municipal Financial Investment Review Center,Tonghua 134000,China;School of Life Science,Changchun Normal University,Changchun 130032,China)

机构地区：[1]长春师范大学中心实验室,吉林长春130032 [2]通化市财政投资评审中心,吉林通化134000 [3]长春师范大学生命科学学院,吉林长春130032

出　　处：《长春师范大学学报》2024年第12期108-112,共5页Journal of Changchun Normal University

基　　金：吉林省教育厅科学研究项目“桑黄多糖抗氧化活性的构效关系研究”(JJKH20230909KJ);长春师范大学自然科学基金项目“桑黄多糖的结构分析及其抗氧化活性研究”(CSJJ2022001ZK);长春师范大学跨学科基金项目“长白山特色小浆果资源综合开发利用”(长师大KXK[2020]第001号)。

摘　　要：以桑树桑黄的子实体为原料,采用单因素和正交实验优化桑黄多糖的酶提取工艺,优化后的多糖最佳提取条件为酶解时间100 min、酶解温度55℃、酶添加量3%。在此条件下提取了桑树桑黄多糖,得率为(4.470±0.025)%。对桑树桑黄多糖进行细胞毒性检测,结果表明,桑树桑黄多糖对细胞没有毒性作用。Using the fruiting bodies of Sanghuangporus sanghuang as raw materials,the enzymatic extraction of polysaccharides was optimized by single-factor and orthogonal experiments.The optimal extraction conditions for polysaccharides were enzymatic hydrolysis time of 100 min,enzymatic hydrolysis temperature of 55℃,and enzyme addition amount of 3%.Under these conditions,the polysaccharides of Sanghuangporus sanghuang were extracted,with a yield of(4.470±0.025)%.The cytotoxicity test of the polysaccharides indicated that the polysaccharides had no toxic effects on cells.

关 键 词：桑树桑黄 多糖 提取工艺 细胞毒性 

分 类 号：R284.2[医药卫生—中药学] Q-33[医药卫生—中医学]