稀土荧光探针用于快速检测乙肝表面抗原  

Rare Earth Fluorescent Probes for Rapid Detection of Hepatitis B Surface Antigen

作　　者：张瑞起 常宇[2] 刘作家[2] 李雪松 潘利华[2] ZHANG Rui-Qi;CHANG Yu;LIU Zuo-Jia;LI Xue-Song*;PAN Li-Hua*(Ministry of Education Key Laboratory of Advanced Structural Materials,College of Materials Science and Engineering,Changchun University of Technology,Changchun 130012,China;Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]长春工业大学材料科学与工程学院,先进结构材料教育部重点实验室,长春130012 [2]中国科学院长春应用化学研究所,长春130022

出　　处：《应用化学》2024年第12期1760-1769,共10页Chinese Journal of Applied Chemistry

基　　金：新疆维吾尔自治区科技厅重点研发项目(No.2021B03003);吉林省科技厅重点研发项目(No.20224094094XY);吉林省自然科学基金面上项目(No.20230101330JC)资助。

摘　　要：采用自合成的稀土螯合剂4,4′-双(1″,1″,1″,2″,2″,3″,3″-7氟-4″,6″-己二酮-6″-基)氯磺化三联苯(BHHCT)制备乙肝表面抗体标记物,应用侧流免疫层析试纸条和配套的时间分辨荧光检测系统,建立了一种时间分辨荧光免疫分析对乙肝表面抗原(HBsAg)快速检测的方法。该方法线性范围为0.2~500 ng/mL,检出限为0.16 ng/mL,线性回归决定系数(R^(2))为0.9996。批内变异系数小于5%,批间变异系数小于8%,样品回收率94.3%~107.8%。该方法操作简便快捷,20 min内获得检测结果,具有精准、快速、特异性强和无需专业人员操作等优势,满足了乙肝表面抗原的即时检测的精准、快速要求。In this study,a self-synthesized rare-earth chelator 4,4′-Bis(1″,1″,1″,2″,2″,3″,3″-7-fluoro-4″,6″-hexanedione-6″-yl)chlorosulfonated terphenyl(BHHCT)was used to prepare hepatitis B surface antibody markers,and a time-resolved fluorescence immunoassay for the rapid detection of hepatitis B surface antigen(HBsAg)was developed by applying lateral-flow immunochromatographic test strips and an accompanying time-resolved fluorescence detection system.The linear range was 0.2~500 ng/mL,the detection limit was 0.16 ng/mL,and the linear regression coefficient of determination(R^(2))was 0.9996.The intra-batch coefficient of variation was less than 5%,the inter-batch coefficient of variation was less than 8%,and the recoveries of the samples ranged from 94.3%to 107.8%.The method has the advantages of precision,rapidity,specificity and no need for specialized personnel to operate,which can meet the requirement of immediate detection of hepatitis B surface antigen.

关 键 词：时间分辨荧光免疫分析法 侧流免疫层析试纸条 乙肝表面抗原 即时检测 

分 类 号：O655[理学—分析化学]