不同炮制方法对益母草化学成分及活血化瘀作用的影响  

Effects of different processing methods on chemical components and promoting blood circulation and removing blood stasis of Leonuri Herba

作　　者：唐木聪 卢泳 甘国兴 邓桂珠 龙国斌 宋飞 陈小锐 陈海明 TANG Mu-cong;LU Yong;GAN Guo-xing;DENG Gui-zhu;LONG Guo-bin;SONG Fei;CHEN Xiao-rui;CHEN Hai-ming(The Affilited Qingyuan Hospital(Qingyuan People′s Hospital),Guangzhou Medical University,Qingyuan 511518,China;Qingyuan Hospital of Traditional Chinese Medicine;Engineering Technology Research Center of Fresh Medicine and National Medicine of Guangdong Province,Qingyuan 511500,China;Food and Drug Inspection Institute of Qingyuan,Qingyuan 511520,China)

机构地区：[1]广州医科大学附属清远医院(清远市人民医院),清远511518 [2]清远市中医院 [3]广东省鲜药民族医药工程技术研究中心,清远511500 [4]清远市食品药品检验所,清远511520

出　　处：《天然产物研究与开发》2025年第1期114-121,142,共9页Natural Product Research and Development

基　　金：广东省中医药局科研项目(20221464)。

摘　　要：研究不同炮制方法对益母草化学成分及活血化瘀作用的影响,为合理用药提供指导。选择盐酸益母草碱、盐酸水苏碱、芦丁、金丝桃苷、异槲皮苷为指标,评价醋制、酒制、四制对益母草生物碱类和黄酮类成分的影响;采用急性血瘀大鼠模型,以凝血酶时间、纤维蛋白原、全血高切粘度、全血中切粘度、全血低切粘度、血浆粘度、全血高切还原粘度、全血中切还原粘度、全血低切还原粘度为指标,评价醋制、酒制、四制对益母草活血化瘀作用的影响。结果显示不同方法炮制后益母草中盐酸益母草碱、盐酸水苏碱等生物碱类成分含量略有下降,芦丁、金丝桃苷、异槲皮苷等黄酮类成分含量明显下降;不同方法炮制后益母草能不同程度改善模型大鼠的血液流变学指标。本研究表明不同炮制方法对益母草生物碱类成分的影响较小,对黄酮类成分的影响较大;不同炮制方法均能不同程度增强益母草的活血化瘀作用。The effect of different processing methods on the chemical components of Leonuri Herba and the action of Leonuri Herba on promoting blood circulation and removing blood stasis was studied to provide guidance for rational use of drugs.The leonurine hydrochloride,stachydrine hydrochloride,rutin,hyperin and isoquercitrin were chose as the index to evaluate the effect of different processing methods on the alkaloid components and flavonoid components in Leonuri Herba.The thrombin time,fibrinogen,whole blood high shear viscosity,whole blood mid shear viscosity,whole blood low shear viscosity,whole blood high shear reduced viscosity,whole blood mid shear reduced viscosity,whole blood low shear reduced viscosity and plasma viscosity were chose as the index to evaluate the effect of different processing methods on the action of Leonuri Herba on promoting blood circulation and removing blood stasis based on acute blood stasis model rats.The results show that the contents of alkaloid components such as leonurine hydrochloride and stachydrine hydrochloride in Leonuri Herba were decreased slightly compared with before processing,and the flavonoid components such as rutin,hyperin and isoquercitrin were decreased significantly.Leonuri Herba processed with different methods could differently improve the hemorheology index of acute blood stasis model rats.This study showed that different processing methods had a little effect on the alkaloid components of Leonuri Herba,but had great effect on the flavonoid components.Different processing methods could differently improve the action of Leonuri Herba on promoting blood circulation and removing blood stasis.

关 键 词：炮制方法 益母草 化学成分 活血化瘀 

分 类 号：R283.1[医药卫生—中药学]