中国主产区冬虫夏草菌ITS基因及其寄主蝙蝠蛾COI基因的系统发育学分析  

Phylogenetic Analysis of the ITS Gene of Cordyceps sinensis and the COI Gene of Its Host Hepiaua larva in the Main Production Areas of China

作　　者：杜玉杰 李劲涛[2] 陈玉龙 DU Yujie;LI Jintao;CHEN Yulong(Zhanjiang Preschool Education College(College of Basic Education,Lingnan Normal University),Zhanjiang 524037,Guangdong,China;School of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China;Institute of Resources,Ecology and Environment,Sichuan Academy of Forestry,Chengdu 610081,Sichuan,China)

机构地区：[1]湛江幼儿师范专科学校(岭南师范学院基础教育学院),广东湛江524037 [2]北京工业大学生命科学与生物工程学院,北京100124 [3]四川省林科院资源生态与环境研究所,四川成都610081

出　　处：《西昌学院学报(自然科学版)》2024年第4期1-11,共11页Journal of Xichang University(Natural Science Edition)

基　　金：广东省普通高校特色创新类项目(2022KTSCX350);北京市教委科技计划一般项目(KM201910005004);四川省财政专项(ZL2017-06);湛江市工程技术研究中心项目(2022A115)。

摘　　要：[目的]对冬虫夏草菌ITS基因序列及其寄主蝙蝠蛾COI基因序列进行系统发育学分析,研究其遗传分化和变异程度。[方法]采用系统发育分析法,分析ITS基因、COI基因序列并构建系统发育树,计算序列单倍型多样性(Hd)、核苷酸多态性(Pi)。[结果]冬虫夏草菌ITS基因序列可分为17个单倍型,Hd=0.875,Pi=0.00119;寄主蝙蝠蛾COI基因序列可分为18个单倍型,Hd=1.000,Pi=0.00034。利用基因替代速率推测冬虫夏草菌至少起源于58.3百万年前,寄主蝙蝠蛾约起源于17.5百万年前。[结论]冬虫夏草菌及其寄主蝙蝠蛾的遗传差异均与所处纬度无关,且遗传距离与地理距离无相关性;中国主产区冬虫夏草菌及其寄主蝙蝠蛾群体具有丰富的遗传和分化多样性,可能是冬虫夏草菌及其寄主蝙蝠蛾的起源之一;冬虫夏草菌的分化时间明显早于寄主蝙蝠蛾,推测冬虫夏草菌在寄生蝙蝠蛾之前可能以土生方式存在或者有另外的寄主,在寄生蝙蝠蛾后二者产生了协同进化关系,并促使寄主蝙蝠蛾的多样性分化。[Objective]To analyse the ITS gene sequence of Cordyceps sinensis and the COI gene sequence of its host Hepi-aua larva so as to study their genetic differentiation and variation degree.[Method]Using phylogenetic analysis,the phylogeny trees were constructed based on the analyses of ITS gene and COI gene,and haplotype diversity(Hd)and nucleotide diversity(Pi)of the sequences were calculated.[Result]The ITS gene sequences of Cordyceps sinensis could be divided into 17 haplotypes,with Hd=0.875 and Pi=0.00119.The COI gene sequences of Hepiaua larva could be divided into 18 haplotypes,with Hd=1.000 and Pi=0.00034.Using the gene replacement rate,it was estimated that Cordyceps si-nensis dated back to at least 58.3 million years ago while the Hepiaua larva originated from about 17.5 million years ago.[Conclusion]The genetic differences between Cordyceps sinensis and its host Hepiaua larva are both unrelated to the latitude where they are located and their genetic distances show no correlation with their geographical distances.The Cordy-ceps sinensis and its host Hepiaua larva possess rich genetic and differentiation diversities in the main production areas of China,which may be one of the origins of them.The differentiation time of Cordyceps sinensis was significantly earlier than that of Hepiaua larva,and it was speculated that Cordyceps sinensis might have existed in an indigenous way or had other hosts before parasitizing Hepiaua larva.After parasitizing Hepiaua larva,a co-evolutionary relationship was formed between them,which promoted the diversity and differentiation of the Hepiaua larva.

关 键 词：冬虫夏草菌 蝙蝠蛾 ITS基因 COI基因 系统发育 遗传差异 

分 类 号：S567.35[农业科学—中草药栽培]