大连地区健康成年人群外周血T淋巴细胞15种免疫表型分析研究  

Analysis of 15 Immunophenotypes of Peripheral Blood T Lymphocytes in Dalian Area

作　　者：许兆杰 吴京学 王娇[2] 田瑶 汤亚微 朱杰[2] XU Zhaojie;WU Jingxue;WANG Jiao;TIAN Yao;TANG Yawei;ZHU Jie(Department of Clinical Laboratory,the Second Hospital of Dalian Medical University,Liaoning Dalian 116023,China;Department of Flow Cytometry Center,the Second Hospital of Dalian Medical University,Liaoning Dalian 116023,China)

机构地区：[1]大连医科大学附属第二医院检验科,辽宁大连116023 [2]大连医科大学附属第二医院流式细胞中心,辽宁大连116023

出　　处：《现代检验医学杂志》2025年第1期1-6,12,共7页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金项目(82302047);辽宁省博士科研启动基金(2023-BS-160);大连医科大学附属第二医院“1+X”计划临床技术水平提升优势技术项目(2022LCJSYS03)。

摘　　要：目的 分析大连地区健康成年人外周血T淋巴细胞15种免疫表型特征与年龄及性别之间的关系。方法 选取2022年10月~2023年6月在大连医科大学附属第二医院体检中心诊疗的277例健康成年人作为研究对象,其中男性154例,女性123例。按不同年龄将研究对象分为三组:青年组(18~44岁,n=103)、中年组(45~60岁,n=114)和老年组(>60岁,n=60)。通过流式细胞术检测外周血T淋巴细胞免疫表型,包括初始(N)、中央记忆(CM)、效应记忆(EM)、终末分化(TEM)、活化(HLA-DR^(+))及衰老(CD28^(-))CD4^(+)和CD8^(+)T淋巴细胞绝对计数和百分比,比较不同性别和不同年龄段T淋巴细胞免疫表型绝对计数和百分比的差异性;同时采用Spearman相关性分析评估年龄与T淋巴细胞免疫表型绝对计数和百分比的相关性。结果 与青年组比较,中年组和老年组中CD8^(+)TEM,CD4^(+)HLA-DR^(+),CD8^(+)HLA-DR^(+)和CD8^(+)CD28^(-)T淋巴细胞绝对计数和百分比升高(Z=2.009~6.607),CD8^(+)N T淋巴细胞绝对计数和百分比降低(Z=5.574~7.999),差异具有统计学意义(均P<0.05)。与女性组比较,男性组中CD8^(+)CM,CD8^(+)HLA-DR^(+),CD8^(+)CD28^(-)T淋巴细胞绝对计数和百分比升高(Z=2.945~6.131),CD3^(+),CD4^(+)和CD8^(+)N T淋巴细胞绝对计数和百分比降低(Z=2.075~4.225),差异具有统计学意义(均P<0.05)。Spearman相关性分析表明,年龄与CD4^(+)CM,CD4^(+)HLA-DR^(+),CD4^(+)CD28^(-),CD8^(+)TEM,CD8^(+)HLA-DR^(+)和CD8^(+)CD28^(-)T淋巴细胞绝对计数和百分比呈正相关(r=0.125~0.479,均P<0.05),与CD4^(+)N和CD8^(+)N T淋巴细胞绝对计数和百分比呈负相关(r=-0.538~-0.148,均P<0.05)。结论 大连地区健康成年人外周血T淋巴细胞15种免疫表型受年龄和性别影响,有必要建立适宜当地的参考区间,为机体免疫功能评估提供更精准的参考依据。Objective To analyze the relationship between 15 immunophenotypes of peripheral blood T cells and age and gender in healthy adults in the Dalian area.Methods A total of 277 healthy adults admitted to the physical examination center of the Second Affiliated Hospital of Dalian Medical University from October 2022 to June 2023 were selected as the research subjects,including 154 males and 123 females.They were divided into three groups according to age:young group(18~44 years,n=103),middle-aged group(45~60 years,n=114)and old age group(>60 years,n=60).Flow cytometry was used to determine immunophenotypes of T cells,including the absolute count and proportion of naïve cells(N),central memory(CM),effector memory(EM),terminal effector memory(TEM),activation(HLA-DR^(+))and senescence(CD28^(-))CD4^(+)and CD8^(+)T cells,and the differences of them among age and gender were analyzed.Spearman correlation analysis was also used to evaluate the correlation between age and immunophenotypes of T cells.Results Compared with the young group,the absolute count and proportion of CD8^(+)TEM,CD4^(+)HLA-DR^(+),CD8^(+)HLA-DR^(+)and CD8^(+)CD28^(-)T lymphoaytes cells were increased in the middleaged group and old age group(Z=2.009~6.607),while the absolute count and proportion of CD8^(+)N T cells were decreased in the middle-aged group(Z=5.574~7.999)and old age group,and the differences were statistically significant(all P<0.05),respectively.Compared with the female group,the absolute count and proportion of CD8^(+)CM,CD8^(+)HLA-DR^(+)and CD8^(+)CD28^(-)T cells were increased in the female group(Z=2.945~6.131),while the absolute count and proportion of CD3^(+),CD4^(+)and CD8^(+)N T cells were decreased in the male group(Z=2.075~4.225),and the differences were statistically significant(all P<0.05),respectively.Spearman correlation analysis showed that age was positively correlated with the absolute count and proportion of CD4^(+)CM,CD4^(+)HLA-DR^(+),CD4^(+)CD28^(-),CD8^(+)TEM,CD8^(+)HLA-DR^(+)and CD8^(+)CD28^(-)T lymphocytes cells(r=0.1

关 键 词：T淋巴细胞 免疫表型 流式细胞术 大连地区 

分 类 号：R446.6[医药卫生—诊断学]