子宫内膜异位症患者血清GP73和SMAD2表达水平及临床价值研究  

Study on the Expression Level of Serum GP73 and SMAD2 and Clinical Value in Patients with Endometriosis

作　　者：丁辉[1] 苏雪梅[1] 张蓉[1] DING Hui;SU Xuemei;ZHANG Rong(Department of Obstetrics and Gynecology,Xinjiang Military Region General Hospital,Urumqi 830000,China)

机构地区：[1]新疆军区总医院妇产科,乌鲁木齐830000

出　　处：《现代检验医学杂志》2025年第1期122-126,131,共6页Journal of Modern Laboratory Medicine

摘　　要：目的 探究血清中高尔基体蛋白73(GP73),SMAD家族成员2(SMAD2)在子宫内膜异位症(endometriosis,EMT)中的表达及临床意义。方法 选取2022年3月~2023年9月新疆军区总医院收治的175例EMT患者作为观察组,根据EMT分期将患者分为轻度组(Ⅰ期,n=61)、中度组(Ⅱ期,n=52)和重度组(Ⅲ~Ⅳ期,n=62);选择同期进行体检的163例健康女性作为对照组。采用ELISA法对血清GP73,SMAD2水平进行检测,并对观察组和对照组的一般资料进行比较。采用Logistic回归模型分析患者发生EMT的影响因素。采用Pearson法对EMT患者血清GP73与SMAD2的水平相关性进行分析;ROC曲线评估血清GP73,SMAD2水平以及二者联合对EMT患者的诊断价值。结果两组患者是否存在痛经和月经不调比较,差异具有统计学意义(χ^(2)=17.633,39.268,均P <0.001)。观察组血清GP73(73.68±19.23 ng/ml),SMAD2(42.27±9.61 mg/L)表达水平均高于对照组(58.61±13.27 ng/ml,35.26±6.37mg/L),差异具有统计学意义(t=8.327,7.845,均P <0.05)。轻度组、中度组、重度组血清GP73(59.79±17.26,73.73±18.17l,87.29±22.05 ng/ml)和SMAD2(35.18±7.39,39.97±9.45,51.17±11.96 mg/L)水平依次增加,差异具有统计学意义(F=31.067,42.866,均P <0.05)。Pearson法分析EMT患者血清GP73水平与SMAD2水平呈正相关(r=0.427,P<0.001)。多因素Logistic回归分析显示,月经不调、痛经、血清GP73[OR(95%CI):2.035(1.208~3.428)],SMAD2[OR(95%CI):1.972(1.284~3.029)]水平是患者发生EMT的危险因素(均P <0.05)。血清GP73和SMAD2联合诊断EMT的AUC(95%CI)为0.821(0.776~0.861),高于血清GP73和SMAD2单独诊断[0.763(0.714~0.807),0.708(0.656~0.756)],差异具有统计学意义(Z=3.121,4.346,均P <0.05)。结论 EMT患者血清GP73,SMAD2水平升高,均与患者的病情程度有关,对EMT有一定诊断价值。Objective To investigate the expression and clinical significance of serum Golgi protein 73(GP73)and SMAD family member 2(SMAD2)in endometriosis.Methods From March 2022 to September 2023,175 patients with endometriosis who visited Xinjiang Military Region General Hospital were collected as the observation group.According to the staging of endometriosis,patients were separated into mild group(stage Ⅰ,n=61),moderate group(stage Ⅱ,n=52)and severe group(stage Ⅲ-Ⅳ,n=62).The healthy group comprised 55 healthy individuals who underwent medical examinations from December 2020 to December 2022 at Xinjiang Military Region General Hospital.ELISA was applied to detect serum GP73 and SMAD2 levels,the general data of the observation group and the control group were compared.Logistic regression was used to analyze the relevant factors affecting the occurrence of endometriosis,the Pearson method was applied to investigate the correlation between serum GP73 and SMAD2 levels in patients with endometriosis.ROC curves were used to assess the diagnostic value of serum GP73,SMAD2 levels and their combination in patients with EMT.Results The presence or absence of dysmenorrhea and irregular menstruation was compared between the two groups,and the differences were statistically significant(χ^(2)=17.633,39.268,all P<0.001).Serum GP73(73.68±19.23 ng/ml),SMAD2(42.27±9.61 mg/L)expression levels in the observation group were higher than those in the control group(58.61±13.27 ng/ml,35.26±6.37 mg/L),and the differences were statistically significant(t=8.327,7.845,all P<0.05).GP73(59.79±17.26,73.73±18.17,87.29±22.05ng/ml)and SMAD2(35.18±7.39,39.97±9.45,51.17±11.96mg/L)increased sequentially in the mild,moderate and severe groups,and the differences were statistical significance(F=31.067,42.866,all P<0.05).Pearson analysis showed serum GP73 levels were positively correlated with SMAD2 levels in EMT patients(r=0.427,P<0.001).Multifactorial Logistic regression analysis showed that irregular menstruation,dysmenorrhea,serum GP7

关 键 词：高尔基体蛋白73 SMAD家族成员2 子宫内膜异位症 

分 类 号：R711.71[医药卫生—妇产科学] R392.11[医药卫生—临床医学]