cisAB血型血清学和分子生物学特点及输血策略  

Serological and Molecular Biological Characteristics of cisAB Blood Group and Transfusion Strategies

作　　者：吴思梦[1] 杨巧妮[1] 高娃[1] 李孝帅 王秋实[1] WU Si-Meng;YANG Qiao-Ni;GAO Wa;LI Xiao-Shuai;WANG Qiu-Shi(Department of Blood Transfusion,Shengjing Hospital of China Medical University,Shenyang 110004,Liaoning Province,China)

机构地区：[1]中国医科大学附属盛京医院输血科,辽宁沈阳110004

出　　处：《中国实验血液学杂志》2025年第1期206-210,共5页Journal of Experimental Hematology

基　　金：辽宁省科技厅重点研发计划课题(2021JH2-10300045)。

摘　　要：目的:分析5例cisAB血型样本的血清学及分子生物学特点,并探讨其安全输血方案。方法:使用抗-A、抗-B、抗-D、抗-A1、抗-H定型试剂和ABO试剂红细胞对样本血型进行血清学鉴定;采用PCR-SSP或基因测序对样本血型进行分子生物学测定。结果:5例患者血型血清学鉴定均正反定型不符,表现为A2B3或A2Bw;对样本1、2、3进行ABO基因测序,均存在第6外显子出现261del G,第7外显子发生467 C>T、803 G>C变异,确定样本1、2、3基因型为cis AB/O;样本4、5进行PCR-SSP基因分型,结果均为cis AB/O。结论:cis AB等位基因患者的血清学表现多不一致,有必要进行基因检测,从而确保输血治疗的安全有效性。Objective:To analyze the serological and molecular biological characteristics of 5 patients with cisAB blood group,and to explore the safe transfusion strategy.Methods:Serological identification of the samples′blood group was performed using anti-A,anti-B,anti-D,anti-A1,anti-H typing reagents and ABO reagent erythrocytes.Molecular biological identification of the samples′blood group was performed using PCR-SSP or gene sequencing.Results:The serological identification results of blood group in 5 patients all showed inconsistent forward and reverse typing,presenting as A2B3 or A2Bw.ABO gene sequencing of samples 1,2 and 3 showed 261delG in exon 6 and 467C>T,803G>C in exon 7.The genotypes of samples 1,2 and 3 were determined to be cisAB/O.PCR-SSP genotyping was performed on sample 4 and 5,and the results were both cisAB/O.Conclusion:Patients with cisAB alleles have inconsistent serological manifestations,and genetic testing is necessary to ensure the safety and effectiveness of blood transfusion.

关 键 词：cisAB 血型 血清学 分子生物学 输血策略 

分 类 号：R457.11[医药卫生—治疗学]