温度循环保存对血小板聚集率及常规参数的影响分析  

Effect of Temperature Cycle Preservation on Platelet Aggregation Rate and Routine Parameters

作　　者：梁菊玲 邓智豪[1] 卓创近[1] 黄露[1] 徐静[1] 伍伟健[1] LIANG Ju-Ling;DENG Zhi-Hao;ZHUO Chuang-Jin;HUANG Lu;XU Jing;WU Wei-Jian(Foshan Central Blood Station,Foshan 528000,Guangdong Province,China)

机构地区：[1]佛山市中心血站,广东佛山528000

出　　处：《中国实验血液学杂志》2025年第1期236-240,共5页Journal of Experimental Hematology

摘　　要：目的:对比分析温度循环保存血小板与4℃冷藏保存、22℃振荡保存血小板的聚集率及血小板常规参数变化,为探讨血小板保存方法提供更多实验数据。方法:对温度循环保存血小板、4℃冷藏保存血小板、22℃振荡保存血小板保存d 1、2、3、4、6共5个时间点采集血样,检测其血小板最大聚集率(MAR)及血小板常规参数,包括血小板计数(PLT)、平均血小板体积(MPV)、血小板分布宽度(PDW)和大血小板比率(P-LCR)。结果:3组血小板MAR均随保存时间延长呈显著下降趋势,以22℃组下降最快,4℃组最慢,温度循环组介于两者之间;保存d 3,4℃组MAR仍在正常范围内(MAR>60%),温度循环组约50%,22℃组最低;保存d 4,3组MAR均低于50%,温度循环组MAR显著低于4℃组且高于22℃组(均P<0.05);保存d 6,温度循环组MAR显著低于4℃组(P<0.05),而与22℃组差异无统计学意义(P>0.05)。3组PLT值均随保存时间延长显著下降,保存6 d内3组PLT值均显著低于保存初期(均P<0.05);温度循环组6 d内PDW无显著变化,MPV、P-LCR显著升高;4℃组6 d内MPV、PDW、P-LCR均显著下降,而22℃组显著上升;在相同保存天数下温度循环组PLT值与4℃组、22℃组无明显差异,MPV、PDW、P-LCR值显著高于4℃组、低于22℃组(均P<0.05)。结论:温度循环保存血小板的聚集功能、血小板常规参数变化介于4和22℃保存之间。Objective: To compare and analyze the changes of aggregation rate and routine parameters of platelets stored in temperature cycle, cold storage at 4 ℃ and oscillating storage at 22 ℃, so as to provide more experimental data for platelet preservation methods. Methods: Blood samples were collected at 5 time points on the 1~(st), 2~(nd), 3~(rd), 4~(th) and 6~(th) day after platelet cycling preservation at temperature, cold storage at 4 ℃, and oscillating storage at 22 ℃. Platelet maximum aggregation rate(MAR) and routine parameters including platelet count(PLT), mean platelet volume(MPV), platelet distribution width(PDW) and platelet-larger cell ratio(P-LCR) were detected. Results: The platelet MAR of three groups showed a significant decrease trend with the preservation time, the fastest decrease was in the 22 ℃ group, the slowest was in the 4 ℃ group, and the temperature cycle group was between the two groups. On the 3~(rd) day of preservation, the platelet MAR in 4 ℃ group was still in the normal range(MAR>60%), while in temperature cycle group was about 50%, and in 22 ℃ group was the lowest. On the 4~(th) day of preservation, platelet MAR in all the three groups was lower than 50%, and that in temperature cycle group was significantly lower than in 4 ℃ group but higher than in 22 ℃ group(both P<0.05). On the 6~(th) day of preservation, platelet MAR in the temperature cycle group was significantly lower than that in the 4 ℃ group(P<0.05), but there was no statistically significant difference compared to 22 ℃ group(P>0.05). PLT values in the three groups were all significantly decreased with the preservation time extension, and were significantly lower than those in the early stage of preservation within 6 days(all P<0.05). PDW in temperature cycle group had no significant change within 6 days of preservation, but MPV and P-LCR were significantly increased. MPV, PDW and P-LCR all decreased significantly in 4 ℃ group within 6 days of preservation but increased in 22 ℃ group. Under the sa

关 键 词：温度循环保存 血小板 血小板聚集率 冷藏保存 

分 类 号：R331.143[医药卫生—人体生理学]