不同工艺制备的腐植酸对NaCl胁迫下小麦生长的影响  

Effects of Humic Acids Produced by Different Process on Wheat Growth under NaCl Stress

作　　者：张子怡 王学虎 苑莹 沈志峰 黄志浩 Zhang Ziyi;Wang Xuehu;Yuan Ying;Shen Zhifeng;Huang Zhihao(Hebei Monband Biotechnology Co.Ltd.,Xingtai,0540002;Hebei Monband Water Soluble Fertilizer Co.Ltd.,Shijiazhuang,050000)

机构地区：[1]河北萌帮生物科技有限公司,邢台054000 [2]河北萌帮水溶肥料股份有限公司,石家庄050000

出　　处：《腐植酸》2024年第6期65-70,共6页Humic Acid

基　　金：石家庄市厅市会商专项资金项目(项目编号225490590595A)。

摘　　要：为了探究3种工艺制备的腐植酸(上清褐煤腐植酸、原褐煤腐植酸和发酵褐煤腐植酸)对NaCl胁迫下小麦生长的影响,本试验设5个处理,分别为清水(T1)、150 mmol/L NaCl溶液(T2)、T2+上清褐煤腐植酸溶液(T3)、T2+原褐煤腐植酸溶液(T4)、T2+发酵褐煤腐植酸溶液(T5)。结果表明,与T1相比,T2~T5 NaCl胁迫处理不同程度地抑制了小麦种子萌发和植株生长。在150 mmol/L NaCl溶液(T2)胁迫下,添加3种工艺制备的腐植酸均可显著降低小麦叶片、根部的相对电导率和MDA含量,其中T3处理分别降低22.31%、29.11%、18.70%,T5处理分别降低38.41%、39.29%、21.76%;均可提高小麦的发芽势、发芽率、发芽指数、根长、株高、可溶性糖含量、可溶性蛋白含量以及POD、CAT、SOD活性,其中T3处理的发芽势、发芽率、发芽指数效果最好,分别显著提高了23.25%、28.97%、44.04%;T5处理的可溶性糖含量、可溶性蛋白含量以及POD、CAT、SOD活性效果最好,分别显著提高了37.58%、21.09%、37.63%、35.21%、101.44%;根长、株高差异不显著。综上所述,在促进小麦萌发方面以上清褐煤腐植酸效果显著,在促进小麦幼苗生长、抗氧化能力等方面以发酵褐煤腐植酸效果最好。In order to research the effects of humic acids produced by three processes(supernatant lignite humic acid,original lignite humic acid,and fermented lignite humic acid)on wheat growth under NaCl stress,five treatments were set up in this study.They were five groups,i.e.,clear water(T1),150 mmol/L NaCl solution(T2),T2+supernatant lignite humic acid solution(T3),T2+original lignite humic acid solution(T4),and T2+fermented lignite humic acid solution(T5).The results showed that compared with T1,NaCI stress treatment T2,T3,T4 and T5 all inhibited wheat seed germination and plant growth with varied degrees.Under the stress of 150 mmol/L NaCl solution(T2),the relative conductivity and MDA content of wheat leaves and roots were significantly decreased by adding humic acid prepared by three different processes,and T3 treatment decreased by 22.31%,29.11%and 18.70%,and T5 treatment decreased by 38.41%,39.29%and 21.76%,respectively;The three kinds of humic acid could improve the germination potential,germination rate,germination index,root length,plant height,soluble sugar content,soluble protein content,as well as POD,CAT and SOD activities of wheat.Among them,T3 treatment exhibited the best effect on germination potential,germination rate and germination index,which were significantly increased by 23.25%,28.97%and 44.04%,respectively;T5 treatment showed the best effects on soluble sugar content,soluble protein content,POD,CAT and SOD activities,which were significantly increased by 37.58%,21.09%,37.63%,35.21%and 101.44%,respectively;There was no significant difference in root length and plant height.In summary,the supernatant lignite humic acid had a significant effect on promoting wheat germination,while the fermented lignite humic acid had the best effect on promoting wheat seedling growth and antioxidant capacity.

关 键 词：小麦 腐植酸 NACL胁迫 种子萌发 幼苗生长特性 

分 类 号：TQ314.1[化学工程—高聚物工业] S512.1[农业科学—作物学]