加味天雄散调控精原干细胞增殖的机制研究  

The Mechanism of Modified Tianxiong Powder Regulating Spermatogonial Stem Cell Proliferation

作　　者：陈少峰 李春旭 胡蓆宝[3] 张圆[4] 李耀君[5] 李重[1] 孙远 赵丰 耿强[1] Chen Shaofeng;Li Chunxu;Hu Xibao;Zhang Yuan;LiYaojun;Li Chong;Sun Yuan;Zhao Feng;Geng Qiang(Department of Andrology,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Tianjin Human Sperm Bank,Tianjin Medical University Second Hospital,Tianjin Institute of Urology,Tianjin 300211,China;Department of Gastroenterology,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Department of Pediatrics,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Department of Otolaryngology,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院国家中医针灸临床研究中心男病科,天津300193 [2]天津医科大学第二医院天津市泌尿外科研究所天津市人类精子库,天津300211 [3]天津中医药大学第一附属医院国家中医针灸临床研究中心消化科,天津300193 [4]天津中医药大学第一附属医院国家中医针灸临床研究中心儿科,天津300193 [5]天津中医药大学第一附属医院国家中医针灸临床研究中心耳鼻喉科,天津300193

出　　处：《南开大学学报(自然科学版)》2024年第6期28-32,共5页Journal of Nankai University(Natural Sience)

基　　金：国家自然科学基金面上项目(82474513);国家自然科学基金青年项目(82405406);天津市卫生健康委员会中医中西医结合课题(2021047,2023131,2023183);天津市教育委员会科研计划项目(2023KJ155);中国中医科学院科技创新工程-重大攻关项目(CI2021A02201)。

摘　　要：为了探讨加味天雄散干预SCF-SRC-AKT-FYN-PLCγ信号通路调节精原干细胞增殖的作用机制,将75只清洁级SD大鼠随机分为5组(每组15只),即对照组、模型组、低剂量组、中剂量组和高剂量组.对照组予以等剂量水溶剂灌胃处理,其余4组进行腺嘌呤灌胃建立少精子症大鼠模型.建模结束后,模型组和对照组予以等剂量水溶剂治疗,其余3组分别予以低、中、高剂量加味天雄散治疗.治疗4周后检测大鼠精子浓度和活率,RT-PCR和WB分别检测睾丸组织精原干细胞特异性标志物CDH1和SCF-SRC-AKT-FYN-PLCγ信号通路各因子mRNA和蛋白表达,酶联免疫方法检测氧化应激指标SOD和MDA表达.经加味天雄散治疗后,少精子症大鼠精子浓度和精子活率增加,睾丸组织精原干细胞特异标志物CDH1和SCF-SRC-AKT-FYN-PLCγ信号通路各因子mRNA及其蛋白表达均增加,氧化应激指标SOD表达增加并且MDA表达下降,且差异有统计学意义.本研究表明加味天雄散能够促进精原干细胞增殖,提高精子浓度和精子活率,改善氧化应激,并可能通过调节SCF-SRC-AKT-FYN-PLCγ信号通路促进精原干细胞增殖.To investigate the effect of modified Tianxiong powder on the proliferation of spermatogonial stem cells through SCF-SRC-AKT-FYN-PLCγsignaling pathway.A total of 75 clean SD rats were randomly divided into 5 groups(15 in each group),namely control group,model group,low-dose group,medium-dose group and high-dose group.The control group was given equal dose of aqueous solvent intragastric treatment,and the other 4 groups were given adenine intragastric treatment to establish the rat model of oligospermia.After modeling,the model group and control group were treated with equal dose of aqueous solvent,and the other 3 groups were treated with low,medium and high doses of modified Tianxiong powder respectively.After 4 weeks of treatment,the sperm concentration and motility of the rats were detected,the mRNA and protein expressions of CDH1 and SCF-SRC-AKT-FYN-PLCγsignaling pathway in testicular tissue were detected by PCR and WB,and the expressions of SOD and MDA were detected by enzyme-linked immunoassay.The sperm concentration and sperm motility of the oligospermia rats were increased after treatment with modified Tianxiong powder.After treatment with modified Tianxiong power,the mRNA expression and protein expression of CDH1 and SCF-SRC-AKT-FYN-PLCγsignaling pathway in testicular tissue were increased.After treatment with modified Tianxiong powder,the expression of SOD increased and MDA decreased,and the difference was statistically significant.Modified Tianxiong powder can promote spermatogonial stem cell proliferation,increase sperm concentration and sperm motility,ameliorate oxidative stress,and regulate the proliferation mechanism by SCF-SRC-AKT-FYN-PLCγsignaling pathway.

关 键 词：加味天雄散 精原干细胞增殖 SCF-SRC-AKT-FYN-PLCγ信号通路 氧化应激 

分 类 号：R96[医药卫生—药理学]